Human ProHAMP ELISA Kit

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  • Catalog
    E018427
  • species
    Human
  • GeneProHAMP
  • Standard CurveHuman ProHAMP ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural human Hepcidin prohormone. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human Hepcidin prohormone is typically less than 0.078 ng/mL.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero.
  • Intended UseHuman ProHAMP ELISA Kit allows for the in vitro quantitative determination of ProHAMP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepcidin prohormone. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Hepcidin prohormone and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Hepcidin prohormone, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Hepcidin prohormone in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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