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Human HSPA4 ELISA Kit

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  • Alternative name

    Human hsp70RY ELISA Kit;Human heat shock 70-related protein APG-2 ELISA Kit;Human APG2 ELISA Kit;Human APG-2 ELISA Kit;Human HEL-S-5a ELISA Kit;Human HS24/P52 ELISA Kit;Human HSPH2 ELISA Kit;Human RY ELISA Kit;Human hsp70 ELISA Kit;Human heat shock protein family A (Hsp70) member 4 ELISA Kit;Human heat shock 70 kDa protein 4 ELISA Kit;Human epididymis secretory sperm binding protein Li 5a ELISA Kit;Human heat shock 70kD protein 4 ELISA Kit;Human heat shock 70kDa protein 4 ELISA Kit;Human heat shock protein, 110 kDa ELISA Kit;Human hsp70 RY ELISA Kit;
  • Catalog
    E017966
  • species
    Human
  • GeneHSPA4
  • Other Species Mouse Hspa4 ELISA KitCanine HSPA4 ELISA KitRat Hspa4 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman HSPA4 ELISA Kit allows for the in vitro quantitative determination of HSPA4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Intended Uses: This HSPA4 ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofHSPA4 in the sample, thisHSPA4 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusHSPA4 concentration. The concentration ofHSPA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve Principle of the Assay: This HSPA4 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forHSPA4. Standards or samples are then added to the microtiter plate wells andHSPA4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofHSPA4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forHSPA4 are added to each well to "sandwich" theHSPA4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containHSPA4 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
  • Human Heat shock 70 kDa protein 4 Protein information
  • Uniprot ID HSP74_HUMAN
  • Uniprot AC P34932; O95756; Q2TAL4; Q9BUK9;
  • UniGene Hs.90093;
  • GeneID 3308
  • KEGG hsa:3308;
  • Human Heat shock 70 kDa protein 4 Protein SEQUENCE

  • SEQUENCE 840 AA; 94331 MW; 8B690A52A0729C2A CRC64;

    MSVVGIDLGF QSCYVAVARA GGIETIANEY SDRCTPACIS FGPKNRSIGA

    AAKSQVISNA KNTVQGFKRF HGRAFSDPFV EAEKSNLAYD IVQLPTGLTG

    IKVTYMEEER NFTTEQVTAM LLSKLKETAE SVLKKPVVDC VVSVPCFYTD

    AERRSVMDAT QIAGLNCLRL MNETTAVALA YGIYKQDLPA LEEKPRNVVF

    VDMGHSAYQV SVCAFNRGKL KVLATAFDTT LGGRKFDEVL VNHFCEEFGK

    KYKLDIKSKI RALLRLSQEC EKLKKLMSAN ASDLPLSIEC FMNDVDVSGT

    MNRGKFLEMC NDLLARVEPP LRSVLEQTKL KKEDIYAVEI VGGATRIPAV

    KEKISKFFGK ELSTTLNADE AVTRGCALQC AILSPAFKVR EFSITDVVPY

    PISLRWNSPA EEGSSDCEVF SKNHAAPFSK VLTFYRKEPF TLEAYYSSPQ

    DLPYPDPAIA QFSVQKVTPQ SDGSSSKVKV KVRVNVHGIF SVSSASLVEV

    HKSEENEEPM ETDQNAKEEE KMQVDQEEPH VEEQQQQTPA ENKAESEEME

    TSQAGSKDKK MDQPPQAKKA KVKTSTVDLP IENQLLWQID REMLNLYIEN

    EGKMIMQDKL EKERNDAKNA VEEYVYEMRD KLSGEYEKFV SEDDRNSFTL

    KLEDTENWLY EDGEDQPKQV YVDKLAELKN LGQPIKIRFQ ESEERPKLFE

    ELGKQIQQYM KIISSFKNKE DQYDHLDAAD MTKVEKSTNE AMEWMNNKLN

    LQNKQSLTMD PVVKSKEIEA KIKELTSTCS PIISKPKPKV EPPKEEQKNA

    EQNGPVDGQG DNPGPQAAEQ GTDTAVPSDS DKKLPEMDID

  • UCSC uc003kyj.4; human. [P34932-1];


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