Human ABCG1 ELISA Kit

Alternative name
Human ATP-binding cassette transporter 8 ELISA Kit;Human White protein homolog ELISA Kit;Human ABC8 ELISA Kit;Human WHT1 ELISA Kit;Human WHITE1 ELISA Kit;Human ATP binding cassette subfamily G member 1 ELISA Kit;Human ATP-binding cassette sub-family G member 1 ELISA Kit;Human ABC transporter 8 ELISA Kit;Human ATP-binding cassette transporter member 1 of subfamily G ELISA Kit;Human ATP-binding cassette, sub-family G (WHITE), member 1 ELISA Kit;Human homolog of Drosophila white ELISA Kit;Human white protein homolog (ATP-binding cassette transporter 8) ELISA Kit;
Catalog
E004016
species
Human
GeneABCG1
Standard Curve
Other Species Mouse Abcg1 ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ABCG1 ELISA Kit allows for the in vitro quantitative determination of ABCG1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalPrinciple of the Assay: ABCG1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ABCG1. Standards or samples are then added to the microtiter plate wells and ABCG1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ABCG1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for ABCG1 are added to each well to "sandwich" the ABCG1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ABCG1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ABCG1 concentration in each sample is interpolated from this standard curve.
Human ATP-binding cassette sub-family G member 1 Protein information
Uniprot ID ABCG1_HUMAN
Uniprot AC Q9BXL1; Q9BXL2; Q9BXL3; Q9BXL4;
UniGene Hs.124649; Hs.418279;
GeneID 9619
KEGG hsa:9619;
Human ATP-binding cassette sub-family G member 1 Protein SEQUENCE
SEQUENCE 678 AA; 75592 MW; B901CABDA6C19E09 CRC64;
MACLMAAFSV GTAMNASSYS AEMTEPKSVC VSVDEVVSSN MEATETDLLN
GHLKKVDNNL TEAQRFSSLP RRAAVNIEFR DLSYSVPEGP WWRKKGYKTL
LKGISGKFNS GELVAIMGPS GAGKSTLMNI LAGYRETGMK GAVLINGLPR
DLRCFRKVSC YIMQDDMLLP HLTVQEAMMV SAHLKLQEKD EGRREMVKEI
LTALGLLSCA NTRTGSLSGG QRKRLAIALE LVNNPPVMFF DEPTSGLDSA
SCFQVVSLMK GLAQGGRSII CTIHQPSAKL FELFDQLYVL SQGQCVYRGK
VCNLVPYLRD LGLNCPTYHN PADFVMEVAS GEYGDQNSRL VRAVREGMCD
SDHKRDLGGD AEVNPFLWHR PSEEVKQTKR LKGLRKDSSS MEGCHSFSAS
CLTQFCILFK RTFLSIMRDS VLTHLRITSH IGIGLLIGLL YLGIGNEAKK
VLSNSGFLFF SMLFLMFAAL MPTVLTFPLE MGVFLREHLN YWYSLKAYYL
AKTMADVPFQ IMFPVAYCSI VYWMTSQPSD AVRFVLFAAL GTMTSLVAQS
LGLLIGAAST SLQVATFVGP VTAIPVLLFS GFFVSFDTIP TYLQWMSYIS
YVRYGFEGVI LSIYGLDRED LHCDIDETCH FQKSEAILRE LDVENAKLYL
DFIVLGIFFI SLRLIAYFVL RYKIRAER
UCSC uc002zan.3; human. [P45844-1];

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Human ABCG1 ELISA Kit