Human GUCA2A ELISA Kit

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  • Alternative name

    Human guanylate cyclase activator 2A ELISA Kit;Human guanylate cyclase-activating protein 1 ELISA Kit;Human guanylate cyclase-activating protein I ELISA Kit;Human GCAP-I ELISA Kit;Human GUCA2 ELISA Kit;Human STARA ELISA Kit;Human guanylin ELISA Kit;Human guanylate cyclase activator 2A (guanylin 2, intestinal, heat-stable) ELISA Kit;Human prepro-guanylin ELISA Kit;

  • Catalog
    E017793
  • species
    Human
  • GeneGUCA2A
  • Standard CurveHuman GUCA2A ELISA Kit
  • Other Species Mouse Guca2a ELISA KitGuinea Pig GUCA2A ELISA KitPorcine GUCA2A ELISA KitRat Guca2a ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GUCA2A. No significant cross-reactivity or interference between GUCA2A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GUCA2A and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman GUCA2A ELISA Kit allows for the in vitro quantitative determination of GUCA2A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the assay: GUCA2A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GUCA2A antibody and an GUCA2A-HRP conjugate. The assay sample and buffer are incubated together with GUCA2A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GUCA2A concentration since GUCA2A from samples and GUCA2A-HRP conjugate compete for the anti-GUCA2A antibody binding site. Since the number of sites is limited, as more sites are occupied by GUCA2A from the sample, fewer sites are left to bind GUCA2A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GUCA2A concentration in each sample is interpolated from this standard curve.
  • Human Guanylin Protein information
  • Uniprot ID GUC2A_HUMAN
  • Uniprot AC Q02747;
  • UniGene Hs.778;
  • GeneID 2980
  • KEGG hsa:2980;
  • Human Guanylin Protein SEQUENCE
  • SEQUENCE 115 AA; 12388 MW; C644DA910EED26FA CRC64;

    MNAFLLSALC LLGAWAALAG GVTVQDGNFS FSLESVKKLK DLQEPQEPRV

    GKLRNFAPIP GEPVVPILCS NPNFPEELKP LCKEPNAQEI LQRLEEIAED

    PGTCEICAYA ACTGC

  • UCSC uc001chd.2; human.;


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