Human MX1 ELISA Kit

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  • Alternative name

    Human interferon-induced protein p78 ELISA Kit;Human IFI-78K ELISA Kit;Human interferon-regulated resistance GTP-binding protein MxA ELISA Kit;Human myxoma resistance protein 1 ELISA Kit;Human Myxovirus resistance protein 1 ELISA Kit;Human IFI78 ELISA Kit;Human MX ELISA Kit;Human MxA ELISA Kit;Human MX dynamin like GTPase 1 ELISA Kit;Human interferon-induced GTP-binding protein Mx1 ELISA Kit;Human interferon-inducible protein p78 ELISA Kit;Human myxovirus (influenza virus) resistance 1, interferon-inducible protein p78 ELISA Kit;

  • Catalog
    E020302
  • species
    Human
  • GeneMX1
  • Standard CurveHuman MX1 ELISA Kit
  • Other Species Mouse Mx1 ELISA KitBovine MX1 ELISA KitCanine MX1 ELISA KitHorse MX1 ELISA KitPorcine MX1 ELISA KitRat Mx1 ELISA KitSheep MX1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MX1. No significant cross-reactivity or interference between MX1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MX1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman MX1 ELISA Kit allows for the in vitro quantitative determination of MX1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the assay: MX1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MX1 antibody and an MX1-HRP conjugate. The assay sample and buffer are incubated together with MX1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MX1 concentration since MX1 from samples and MX1-HRP conjugate compete for the anti-MX1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MX1 from the sample, fewer sites are left to bind MX1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MX1 concentration in each sample is interpolated from this standard curve.
  • Human Interferon-induced GTP-binding protein Mx1, N-terminally processed Protein information
  • Uniprot ID MX1_HUMAN
  • Uniprot AC C9JZS6; D3DSI8; Q86YP5; Q96CI3;
  • UniGene Hs.517307;
  • GeneID 4599
  • KEGG hsa:4599;
  • Human Interferon-induced GTP-binding protein Mx1, N-terminally processed Protein SEQUENCE
  • SEQUENCE 662 AA; 75520 MW; 626A7DD946F89384 CRC64;

    MVVSEVDIAK ADPAAASHPL LLNGDATVAQ KNPGSVAENN LCSQYEEKVR

    PCIDLIDSLR ALGVEQDLAL PAIAVIGDQS SGKSSVLEAL SGVALPRGSG

    IVTRCPLVLK LKKLVNEDKW RGKVSYQDYE IEISDASEVE KEINKAQNAI

    AGEGMGISHE LITLEISSRD VPDLTLIDLP GITRVAVGNQ PADIGYKIKT

    LIKKYIQRQE TISLVVVPSN VDIATTEALS MAQEVDPEGD RTIGILTKPD

    LVDKGTEDKV VDVVRNLVFH LKKGYMIVKC RGQQEIQDQL SLSEALQREK

    IFFENHPYFR DLLEEGKATV PCLAEKLTSE LITHICKSLP LLENQIKETH

    QRITEELQKY GVDIPEDENE KMFFLIDKVN AFNQDITALM QGEETVGEED

    IRLFTRLRHE FHKWSTIIEN NFQEGHKILS RKIQKFENQY RGRELPGFVN

    YRTFETIVKQ QIKALEEPAV DMLHTVTDMV RLAFTDVSIK NFEEFFNLHR

    TAKSKIEDIR AEQEREGEKL IRLHFQMEQI VYCQDQVYRG ALQKVREKEL

    EEEKKKKSWD FGAFQSSSAT DSSMEEIFQH LMAYHQEASK RISSHIPLII

    QFFMLQTYGQ QLQKAMLQLL QDKDTYSWLL KERSDTSDKR KFLKERLARL

    TQARRRLAQF PG

  • UCSC uc002yzh.5; human. [P20591-1];


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