Human MX1 ELISA Kit

Alternative name
Human interferon-induced protein p78 ELISA Kit;Human IFI-78K ELISA Kit;Human interferon-regulated resistance GTP-binding protein MxA ELISA Kit;Human myxoma resistance protein 1 ELISA Kit;Human Myxovirus resistance protein 1 ELISA Kit;Human IFI78 ELISA Kit;Human MX ELISA Kit;Human MxA ELISA Kit;Human MX dynamin like GTPase 1 ELISA Kit;Human interferon-induced GTP-binding protein Mx1 ELISA Kit;Human interferon-inducible protein p78 ELISA Kit;Human myxovirus (influenza virus) resistance 1, interferon-inducible protein p78 ELISA Kit;
Catalog
E020302
species
Human
GeneMX1
SpecificityThis assay has high sensitivity and excellent specificity for detection of MX1. No significant cross-reactivity or interference between MX1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MX1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman MX1 ELISA Kit allows for the in vitro quantitative determination of MX1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the assay: MX1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MX1 antibody and an MX1-HRP conjugate. The assay sample and buffer are incubated together with MX1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MX1 concentration since MX1 from samples and MX1-HRP conjugate compete for the anti-MX1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MX1 from the sample, fewer sites are left to bind MX1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MX1 concentration in each sample is interpolated from this standard curve.
Human Interferon-induced GTP-binding protein Mx1, N-terminally processed Protein information
Uniprot ID MX1_HUMAN
Uniprot AC C9JZS6; D3DSI8; Q86YP5; Q96CI3;
UniGene Hs.517307;
GeneID 4599
KEGG hsa:4599;
Human Interferon-induced GTP-binding protein Mx1, N-terminally processed Protein SEQUENCE
SEQUENCE 662 AA; 75520 MW; 626A7DD946F89384 CRC64;
MVVSEVDIAK ADPAAASHPL LLNGDATVAQ KNPGSVAENN LCSQYEEKVR
PCIDLIDSLR ALGVEQDLAL PAIAVIGDQS SGKSSVLEAL SGVALPRGSG
IVTRCPLVLK LKKLVNEDKW RGKVSYQDYE IEISDASEVE KEINKAQNAI
AGEGMGISHE LITLEISSRD VPDLTLIDLP GITRVAVGNQ PADIGYKIKT
LIKKYIQRQE TISLVVVPSN VDIATTEALS MAQEVDPEGD RTIGILTKPD
LVDKGTEDKV VDVVRNLVFH LKKGYMIVKC RGQQEIQDQL SLSEALQREK
IFFENHPYFR DLLEEGKATV PCLAEKLTSE LITHICKSLP LLENQIKETH
QRITEELQKY GVDIPEDENE KMFFLIDKVN AFNQDITALM QGEETVGEED
IRLFTRLRHE FHKWSTIIEN NFQEGHKILS RKIQKFENQY RGRELPGFVN
YRTFETIVKQ QIKALEEPAV DMLHTVTDMV RLAFTDVSIK NFEEFFNLHR
TAKSKIEDIR AEQEREGEKL IRLHFQMEQI VYCQDQVYRG ALQKVREKEL
EEEKKKKSWD FGAFQSSSAT DSSMEEIFQH LMAYHQEASK RISSHIPLII
QFFMLQTYGQ QLQKAMLQLL QDKDTYSWLL KERSDTSDKR KFLKERLARL
TQARRRLAQF PG
UCSC uc002yzh.5; human. [P20591-1];

Category

Human MX1 ELISA Kit