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Alternative name
Somatotropin ELISA KIT; Growth hormonegh ELISA KIT;
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Catalog
E017567
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species
Human
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GenehGH
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SpecificityThe only human protein with significant sequence similarities to growth hormone is prolactin. Testing a 200 ng/mL prolactin solution in this assay, no cross reactivity was detected. Further, Pegvisomant (trade name Somavert) a growth hormone analogue and pharmaceutical used in acromegaly therapy has been tested for cross reactivity in different concentrations. Here no significant influence of Somavert was detected (Table 1). But in Growth Horm IGF Res 18 (2008) 526-532 the authors added 100 mg/L pegvisomant to hGH enriched samples containing 2.6 and 10.1 mug/L hGH and measurement results were 154% and 108% of the expected values respectively [9].
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe analytical sensitivity of the hGH SENSITIVE ELISA E022 yields 0.0115 ng/mL (equal to 11.5 pg/mL, equal to 1.15 pg per well; 2x SD of zero standards in 16-fold determination).
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Intended UseHuman hGH ELISA Kit allows for the in vitro quantitative determination of hGH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyDiabetes & Obesity; Cytokines & Cell Signalling; Endocrinology ; Hormones; Diagnostic Assays; Assay Categories
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Product Description
specificalPrinciple of the assay: The Mediagnost hGH SENSITIVE ELISA E022 is a so-called sandwich-assay. It utilizes a specific, high affinity polyclonal rabbit antiserum coated on the wells of a microtiter plate. The hGH in the samples binds quantitatively to the immobilized antiserum. In the following step, the biotinylated antiserum in turn binds hGH. After washing, a streptavidin-peroxidase-enzyme conjugate will be added, which will bind highly specific to the biotin of the antiserum and will catalyze the substrate to change the color quantitatively depending on the hGH level of the sample.
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