Human ALPI ELISA Kit

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  • Alternative name

    Human IAP ELISA Kit;Human intestinal alkaline phosphatase ELISA Kit;Human alkaline phosphatase, intestinal ELISA Kit;Human intestinal-type alkaline phosphatase ELISA Kit;Human Kasahara isozyme ELISA Kit;Human alkaline phosphomonoesterase ELISA Kit;Human glycerophosphatase ELISA Kit;

  • Catalog
    E001383
  • species
    Human
  • GeneALPI
  • Standard CurveHuman ALPI ELISA Kit
  • Other Species Mouse ALPI ELISA KitRat Alpi ELISA KitBovine ALPI ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of iALP. No significant cross-reactivity or interference between iALP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between iALP and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ALPI ELISA Kit allows for the in vitro quantitative determination of ALPI , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: This iALP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human iALP. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: iALP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-iALP antibody and an iALP-HRP conjugate. The assay sample and buffer are incubated together with iALP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the iALP concentration since iALP from samples and iALP-HRP conjugate compete for the anti-iALP antibody binding site. Since the number of sites is limited, as more sites are occupied by iALP from the sample, fewer sites are left to bind iALP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The iALP concentration in each sample is interpolated from this standard curve.
  • Human Intestinal-type alkaline phosphatase Protein information
  • Uniprot ID PPBI_HUMAN
  • Uniprot AC P09923; B2R7Y4; Q53S80; Q9UBV5; Q9UCL2;
  • UniGene Hs.284255; Hs.37009;
  • GeneID 248
  • KEGG hsa:248;
  • Human Intestinal-type alkaline phosphatase Protein SEQUENCE
  • SEQUENCE 528 AA; 56812 MW; 465306BEDF9F0B79 CRC64;

    MQGPWVLLLL GLRLQLSLGV IPAEEENPAF WNRQAAEALD AAKKLQPIQK

    VAKNLILFLG DGLGVPTVTA TRILKGQKNG KLGPETPLAM DRFPYLALSK

    TYNVDRQVPD SAATATAYLC GVKANFQTIG LSAAARFNQC NTTRGNEVIS

    VMNRAKQAGK SVGVVTTTRV QHASPAGTYA HTVNRNWYSD ADMPASARQE

    GCQDIATQLI SNMDIDVILG GGRKYMFPMG TPDPEYPADA SQNGIRLDGK

    NLVQEWLAKH QGAWYVWNRT ELMQASLDQS VTHLMGLFEP GDTKYEIHRD

    PTLDPSLMEM TEAALRLLSR NPRGFYLFVE GGRIDHGHHE GVAYQALTEA

    VMFDDAIERA GQLTSEEDTL TLVTADHSHV FSFGGYTLRG SSIFGLAPSK

    AQDSKAYTSI LYGNGPGYVF NSGVRPDVNE SESGSPDYQQ QAAVPLSSET

    HGGEDVAVFA RGPQAHLVHG VQEQSFVAHV MAFAACLEPY TACDLAPPAC

    TTDAAHPVAA SLPLLAGTLL LLGASAAP

  • UCSC uc002vst.4; human.;




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