Human GZMH ELISA Kit

Alternative name
Human CCP-X ELISA Kit;Human Cathepsin G-like 2 ELISA Kit;Human CTSGL2 ELISA Kit;Human cytotoxic T-lymphocyte proteinase ELISA Kit;Human Cytotoxic serine protease C ELISA Kit;Human CSP-C ELISA Kit;Human CGL2 ELISA Kit;Human CGL-2 ELISA Kit;Human CTLA1 ELISA Kit;Human granzyme H ELISA Kit;Human cathepsin G-like 2, protein h-CCPX ELISA Kit;Human cytotoxic T-lymphocyte-associated serine esterase 1 ELISA Kit;Human cytotoxin serine protease-C ELISA Kit;
Catalog
E017443
species
Human
GeneGZMH
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human GZMH. No significant cross-reactivity or interference between Human GZMH and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity3.9 pg/ml
Intended UseHuman GZMH ELISA Kit allows for the in vitro quantitative determination of GZMH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntroduction: Granzymes are key components of the immune response that play important roles in eliminating host cells infected by intracellular pathogens. Several granzymes are potent inducers of cell death. A total of eight granzymes (A-G and M) have been identified in the mouse, but only five are known in humans (A, B, H, M and granzyme 3), and granzyme H appears to be specifically human. In adenovirus-infected cells in which granzyme B (gzmB) and downstream apoptosis pathways are inhibited, granzyme H directly cleaves the adenovirus DNA-binding protein (DBP), a viral component absolutely required for viral DNA replication. This virus demonstrated that gzmH directly induces an important decay in viral DNA replication. Interestingly, gzmH also cleaves the adenovirus 100K assembly protein, a major inhibitor of gzmB, and relieves gzmB inhibition. Granzyme H has a very high amino acid identity (>90%) with many portions of the granzyme B sequence, particularly near the amino terminus of the molecule despite performing a distinct enzymic function. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to granzyme H. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for granzyme H and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain granzyme H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of granzyme H in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Granzyme H Protein information
Uniprot ID GRAH_HUMAN
Uniprot AC P20718; G3V2C5; Q6XGZ0; Q6XGZ1;
UniGene Hs.348264;
GeneID 2999
KEGG hsa:2999;
Human Granzyme H Protein SEQUENCE
SEQUENCE 246 AA; 27315 MW; CA6A87F3DA5F1E71 CRC64;
MQPFLLLLAF LLTPGAGTEE IIGGHEAKPH SRPYMAFVQF LQEKSRKRCG
GILVRKDFVL TAAHCQGSSI NVTLGAHNIK EQERTQQFIP VKRPIPHPAY
NPKNFSNDIM LLQLERKAKW TTAVRPLRLP SSKAQVKPGQ LCSVAGWGYV
SMSTLATTLQ EVLLTVQKDC QCERLFHGNY SRATEICVGD PKKTQTGFKG
DSGGPLVCKD VAQGILSYGN KKGTPPGVYI KVSHFLPWIK RTMKRL
UCSC uc001wpr.3; human. [P20718-1];

Human GZMH ELISA Kit

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