Human MRPS2 ELISA Kit

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  • Alternative name

    Human MRP-S2 ELISA Kit;Human S2mt ELISA Kit;Human CGI-91 ELISA Kit;Human mitochondrial ribosomal protein S2 ELISA Kit;Human 28S ribosomal protein S2, mitochondrial ELISA Kit;

  • Catalog
    E041872
  • species
    Human
  • GeneMRPS2
  • Standard CurveHuman MRPS2 ELISA Kit
  • Other Species Mouse Mrps2 ELISA KitBovine MRPS2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MRPS2. No significant cross-reactivity or interference between MRPS2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MRPS2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman MRPS2 ELISA Kit allows for the in vitro quantitative determination of MRPS2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: This MRPS2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MRPS2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||MRPS2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MRPS2 antibody and an MRPS2-HRP conjugate. The assay sample and buffer are incubated together with MRPS2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MRPS2 concentration since MRPS2 from samples and MRPS2-HRP conjugate compete for the anti-MRPS2 antibody binding site. Since the number of sites is limited, as more sites are occupied by MRPS2 from the sample, fewer sites are left to bind MRPS2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MRPS2 concentration in each sample is interpolated from this standard curve.
  • Human 28S ribosomal protein S2, mitochondrial Protein information
  • Uniprot ID RT02_HUMAN
  • Uniprot AC Q9Y399; Q5T899; Q9BSQ4;
  • UniGene Hs.382044;
  • GeneID 51116
  • KEGG hsa:51116;
  • Human 28S ribosomal protein S2, mitochondrial Protein SEQUENCE
  • SEQUENCE 296 AA; 33249 MW; E05A248B09AA1A8B CRC64;

    MATSSAALPR ILGAGARAPS RWLGFLGKAT PRPARPSRRT LGSATALMIR

    ESEDSTDFND KILNEPLKHS DFFNVKELFS VRSLFDARVH LGHKAGCRHR

    FMEPYIFGSR LDHDIIDLEQ TATHLQLALN FTAHMAYRKG IILFISRNRQ

    FSYLIENMAR DCGEYAHTRY FRGGMLTNAR LLFGPTVRLP DLIIFLHTLN

    NIFEPHVAVR DAAKMNIPTV GIVDTNCNPC LITYPVPGND DSPLAVHLYC

    RLFQTAITRA KEKRQQVEAL YRLQGQKEPG DQGPAHPPGA DMSHSL

  • UCSC uc004cfv.6; human.;


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