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Catalog
E016972
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species
Human
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GeneHbA1c
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SpecificityThis assay has high sensitivity and excellent specificity for detection of HbA1c. No significant cross-reactivity or interference between HbA1c and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity88.4ng/mL.
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Intended UseHuman HbA1c ELISA Kit allows for the in vitro quantitative determination of HbA1c , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of HbA1c in human plasma and erythrocyte lysates.
Principle of the Assay||This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to HbA1c has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled HbA1c and unlabeled HbA1c (Standards or samples) with the pre-coated antibody specific to HbA1c. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of HbA1c in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of HbA1c in the sample.
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