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Catalog
E001502
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species
Human
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GeneERAF
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Other Species
Mouse ERAF ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of human SBDP145. No significant cross-reactivity or interference between human SBDP145 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of human ERAF is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
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Intended UseHuman ERAF ELISA Kit allows for the in vitro quantitative determination of ERAF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SBDP145 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SBDP145 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SBDP145 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SBDP145 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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Human Alpha-hemoglobin-stabilizing protein Protein information
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Uniprot ID
Q9NZD4
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Uniprot AC
AHSP_HUMAN
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{Species} Alpha-hemoglobin-stabilizing protein {protein_fuction}
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