Human GRIA1 ELISA Kit

Alternative name
Human gluR-1 ELISA Kit;Human AMPA-selective glutamate receptor 1 ELISA Kit;Human gluR-A ELISA Kit;Human gluR-K1 ELISA Kit;Human Glutamate receptor ionotropic, AMPA 1 ELISA Kit;Human GluA1 ELISA Kit;Human GLUH1 ELISA Kit;Human GLUR1 ELISA Kit;Human GLURA ELISA Kit;Human HBGR1 ELISA Kit;Human glutamate ionotropic receptor AMPA type subunit 1 ELISA Kit;Human glutamate receptor 1 ELISA Kit;Human AMPA 1 ELISA Kit;Human glutamate receptor, ionotropic, AMPA 1 ELISA Kit;
Catalog
E016732
species
Human
GeneGRIA1
SpecificityThis assay has high sensitivity and excellent specificity for detection of GaA. No significant cross-reactivity or interference between GaA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GaA and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman GRIA1 ELISA Kit allows for the in vitro quantitative determination of GRIA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalPrinciple of the assay: GaA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GaA antibody and an GaA-HRP conjugate. The assay sample and buffer are incubated together with GaA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GaA concentration since GaA from samples and GaA-HRP conjugate compete for the anti-GaA antibody binding site. Since the number of sites is limited, as more sites are occupied by GaA from the sample, fewer sites are left to bind GaA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GaA concentration in each sample is interpolated from this standard curve.
Human Glutamate receptor 1 Protein information
Uniprot ID GRIA1_HUMAN
Uniprot AC P42261; B7Z2S0; B7Z2W8; B7Z3F6; B7Z9G9; D3DQI4; E7ESV8; Q2NKM6;
UniGene Hs.519693; Hs.737709;
GeneID 2890
KEGG hsa:2890;
Human Glutamate receptor 1 Protein SEQUENCE
SEQUENCE 906 AA; 101506 MW; 03EA1E026D0A9A2F CRC64;
MQHIFAFFCT GFLGAVVGAN FPNNIQIGGL FPNQQSQEHA AFRFALSQLT
EPPKLLPQID IVNISDSFEM TYRFCSQFSK GVYAIFGFYE RRTVNMLTSF
CGALHVCFIT PSFPVDTSNQ FVLQLRPELQ DALISIIDHY KWQKFVYIYD
ADRGLSVLQK VLDTAAEKNW QVTAVNILTT TEEGYRMLFQ DLEKKKERLV
VVDCESERLN AILGQIIKLE KNGIGYHYIL ANLGFMDIDL NKFKESGANV
TGFQLVNYTD TIPAKIMQQW KNSDARDHTR VDWKRPKYTS ALTYDGVKVM
AEAFQSLRRQ RIDISRRGNA GDCLANPAVP WGQGIDIQRA LQQVRFEGLT
GNVQFNEKGR RTNYTLHVIE MKHDGIRKIG YWNEDDKFVP AATDAQAGGD
NSSVQNRTYI VTTILEDPYV MLKKNANQFE GNDRYEGYCV ELAAEIAKHV
GYSYRLEIVS DGKYGARDPD TKAWNGMVGE LVYGRADVAV APLTITLVRE
EVIDFSKPFM SLGISIMIKK PQKSKPGVFS FLDPLAYEIW MCIVFAYIGV
SVVLFLVSRF SPYEWHSEEF EEGRDQTTSD QSNEFGIFNS LWFSLGAFMQ
QGCDISPRSL SGRIVGGVWW FFTLIIISSY TANLAAFLTV ERMVSPIESA
EDLAKQTEIA YGTLEAGSTK EFFRRSKIAV FEKMWTYMKS AEPSVFVRTT
EEGMIRVRKS KGKYAYLLES TMNEYIEQRK PCDTMKVGGN LDSKGYGIAT
PKGSALRNPV NLAVLKLNEQ GLLDKLKNKW WYDKGECGSG GGDSKDKTSA
LSLSNVAGVF YILIGGLGLA MLVALIEFCY KSRSESKRMK GFCLIPQQSI
NEAIRTSTLP RNSGAGASSG GSGENGRVVS HDFPKSMQSI PCMSHSSGMP
LGATGL
UCSC uc003luy.5; human. [P42261-1];

Category

Human GRIA1 ELISA Kit