Human NPNT ELISA Kit

Alternative name
Human preosteoblast EGF-like repeat protein with MAM domain ELISA Kit;Human Protein EGFL6-like ELISA Kit;Human EGFL6L ELISA Kit;Human POEM ELISA Kit;Human nephronectin ELISA Kit;
Catalog
E025957
species
Human
GeneNPNT
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human NPNT. No significant cross-reactivity or interference between Human NPNT and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.039 ng/ml
Intended UseHuman NPNT ELISA Kit allows for the in vitro quantitative determination of NPNT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NPNT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NPNT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NPNT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NPNT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Nephronectin Protein information
Uniprot ID NPNT_HUMAN
Uniprot AC E9PCK8; E9PCQ1; E9PE64; E9PF04;
UniGene Hs.518921;
GeneID 255743
KEGG hsa:255743;
Human Nephronectin Protein SEQUENCE
SEQUENCE 565 AA; 61907 MW; D4AB4C41B7D5A819 CRC64;
MDFLLALVLV SSLYLQAAAE FDGRWPRQIV SSIGLCRYGG RIDCCWGWAR
QSWGQCQPVC QPRCKHGECI GPNKCKCHPG YAGKTCNQDL NECGLKPRPC
KHRCMNTYGS YKCYCLNGYM LMPDGSCSSA LTCSMANCQY GCDVVKGQIR
CQCPSPGLQL APDGRTCVDV DECATGRASC PRFRQCVNTF GSYICKCHKG
FDLMYIGGKY QCHDIDECSL GQYQCSSFAR CYNIRGSYKC KCKEGYQGDG
LTCVYIPKVM IEPSGPIHVP KGNGTILKGD TGNNNWIPDV GSTWWPPKTP
YIPPIITNRP TSKPTTRPTP KPTPIPTPPP PPPLPTELRT PLPPTTPERP
TTGLTTIAPA ASTPPGGITV DNRVQTDPQK PRGDVFIPRQ PSNDLFEIFE
IERGVSADDE AKDDPGVLVH SCNFDHGLCG WIREKDNDLH WEPIRDPAGG
QYLTVSAAKA PGGKAARLVL PLGRLMHSGD LCLSFRHKVT GLHSGTLQVF
VRKHGAHGAA LWGRNGGHGW RQTQITLRGA DIKSVVFKGE KRRGHTGEIG
LDDVSLKKGH CSEER
UCSC uc003hya.4; human. [Q6UXI9-1];

Category

Human NPNT ELISA Kit

Human preosteoblast EGF-like repeat protein with MAM domain ELISA Kit;Human Protein EGFL6-like ELISA Kit;Human EGFL6L ELISA Kit;Human POEM ELISA Kit;Human nephronectin ELISA Kit;