Human GATA1 ELISA Kit

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  • Alternative name

    Human ERYF1 ELISA Kit;Human GATA-binding factor 1 ELISA Kit;Human GATA-1 ELISA Kit;Human GF-1 ELISA Kit;Human NF-E1 DNA-binding protein ELISA Kit;Human GF1 ELISA Kit;Human NF-E1 ELISA Kit;Human NFE1 ELISA Kit;Human XLANP ELISA Kit;Human XLTDA ELISA Kit;Human XLTT ELISA Kit;Human GATA binding protein 1 ELISA Kit;Human erythroid transcription factor ELISA Kit;Human erythroid transcription factor 1 ELISA Kit;Human globin transcription factor 1 ELISA Kit;Human nuclear factor, erythroid 1 ELISA Kit;Human transcription factor GATA1 ELISA Kit;

  • Catalog
    E016246
  • species
    Human
  • GeneGATA1
  • Standard CurveHuman GATA1 ELISA Kit
  • Other Species Mouse Gata1 ELISA KitChicken GATA1 ELISA KitRat Gata1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GATA1. No significant cross-reactivity or interference between GATA1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GATA1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman GATA1 ELISA Kit allows for the in vitro quantitative determination of GATA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This GATA1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GATA1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: GATA1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GATA1 antibody and an GATA1-HRP conjugate. The assay sample and buffer are incubated together with GATA1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GATA1 concentration since GATA1 from samples and GATA1-HRP conjugate compete for the anti-GATA1 antibody binding site. Since the number of sites is limited, as more sites are occupied by GATA1 from the sample, fewer sites are left to bind GATA1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GATA1 concentration in each sample is interpolated from this standard curve.
  • Human Erythroid transcription factor Protein information
  • Uniprot ID GATA1_HUMAN
  • Uniprot AC P15976; Q96GB8;
  • UniGene Hs.765;
  • GeneID 2623
  • KEGG hsa:2623;
  • Human Erythroid transcription factor Protein SEQUENCE
  • SEQUENCE 413 AA; 42751 MW; 822BD2DE14B908AD CRC64;

    MEFPGLGSLG TSEPLPQFVD PALVSSTPES GVFFPSGPEG LDAAASSTAP

    STATAAAAAL AYYRDAEAYR HSPVFQVYPL LNCMEGIPGG SPYAGWAYGK

    TGLYPASTVC PTREDSPPQA VEDLDGKGST SFLETLKTER LSPDLLTLGP

    ALPSSLPVPN SAYGGPDFSS TFFSPTGSPL NSAAYSSPKL RGTLPLPPCE

    ARECVNCGAT ATPLWRRDRT GHYLCNACGL YHKMNGQNRP LIRPKKRLIV

    SKRAGTQCTN CQTTTTTLWR RNASGDPVCN ACGLYYKLHQ VNRPLTMRKD

    GIQTRNRKAS GKGKKKRGSS LGGTGAAEGP AGGFMVVAGG SGSGNCGEVA

    SGLTLGPPGT AHLYQGLGPV VLSGPVSHLM PFPGPLLGSP TGSFPTGPMP

    PTTSTTVVAP LSS

  • UCSC uc004dkq.5; human. [P15976-1];


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