Human PLCB3 ELISA Kit

Alternative name
Human phosphoinositide phospholipase C-beta-3 ELISA Kit;Human Phospholipase C-beta-3 ELISA Kit;Human PLC-beta-3 ELISA Kit;Human phospholipase C beta 3 ELISA Kit;Human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 ELISA Kit;Human PLC beta 3 ELISA Kit;Human phospholipase C, beta 3 (phosphatidylinositol-specific) ELISA Kit;
Catalog
E029216
species
Human
GenePLCB3
SpecificityThis assay has high sensitivity and excellent specificity for detection of human PLCB3. No significant cross-reactivity or interference between human PLCB3 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human PLCB3 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman PLCB3 ELISA Kit allows for the in vitro quantitative determination of PLCB3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrincple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLCB3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLCB3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLCB3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLCB3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 Protein information
Uniprot ID PLCB3_HUMAN
Uniprot AC Q01970; A5PKZ6; G5E960; Q8N1A4;
UniGene Hs.523761;
GeneID 5331
KEGG hsa:5331;
Human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 Protein SEQUENCE
SEQUENCE 1234 AA; 138799 MW; C5106EFBA8037788 CRC64;
MAGAQPGVHA LQLEPPTVVE TLRRGSKFIK WDEETSSRNL VTLRVDPNGF
FLYWTGPNME VDTLDISSIR DTRTGRYARL PKDPKIREVL GFGGPDARLE
EKLMTVVSGP DPVNTVFLNF MAVQDDTAKV WSEELFKLAM NILAQNASRN
TFLRKAYTKL KLQVNQDGRI PVKNILKMFS ADKKRVETAL ESCGLKFNRS
ESIRPDEFSL EIFERFLNKL CLRPDIDKIL LEIGAKGKPY LTLEQLMDFI
NQKQRDPRLN EVLYPPLRPS QARLLIEKYE PNQQFLERDQ MSMEGFSRYL
GGEENGILPL EALDLSTDMT QPLSAYFINS SHNTYLTAGQ LAGTSSVEMY
RQALLWGCRC VELDVWKGRP PEEEPFITHG FTMTTEVPLR DVLEAIAETA
FKTSPYPVIL SFENHVDSAK QQAKMAEYCR SIFGDALLIE PLDKYPLAPG
VPLPSPQDLM GRILVKNKKR HRPSAGGPDS AGRKRPLEQS NSALSESSAA
TEPSSPQLGS PSSDSCPGLS NGEEVGLEKP SLEPQKSLGD EGLNRGPYVL
GPADREDEEE DEEEEEQTDP KKPTTDEGTA SSEVNATEEM STLVNYIEPV
KFKSFEAARK RNKCFEMSSF VETKAMEQLT KSPMEFVEYN KQQLSRIYPK
GTRVDSSNYM PQLFWNVGCQ LVALNFQTLD VAMQLNAGVF EYNGRSGYLL
KPEFMRRPDK SFDPFTEVIV DGIVANALRV KVISGQFLSD RKVGIYVEVD
MFGLPVDTRR KYRTRTSQGN SFNPVWDEEP FDFPKVVLPT LASLRIAAFE
EGGKFVGHRI LPVSAIRSGY HYVCLRNEAN QPLCLPALLI YTEASDYIPD
DHQDYAEALI NPIKHVSLMD QRARQLAALI GESEAQAGQE TCQDTQSQQL
GSQPSSNPTP SPLDASPRRP PGPTTSPAST SLSSPGQRDD LIASILSEVA
PTPLDELRGH KALVKLRSRQ ERDLRELRKK HQRKAVTLTR RLLDGLAQAQ
AEGRCRLRPG ALGGAADVED TKEGEDEAKR YQEFQNRQVQ SLLELREAQV
DAEAQRRLEH LRQALQRLRE VVLDANTTQF KRLKEMNERE KKELQKILDR
KRHNSISEAK MRDKHKKEAE LTEINRRHIT ESVNSIRRLE EAQKQRHDRL
VAGQQQVLQQ LAEEEPKLLA QLAQECQEQR ARLPQEIRRS LLGEMPEGLG
DGPLVACASN GHAPGSSGHL SGADSESQEE NTQL
UCSC uc009ypg.3; human. [Q01970-1];

Category

Human PLCB3 ELISA Kit