Human FZD3 ELISA Kit

Alternative name
Human Fz-3 ELISA Kit;Human hFz3 ELISA Kit;Human frizzled class receptor 3 ELISA Kit;Human frizzled-3 ELISA Kit;Human frizzled 3, seven transmembrane spanning receptor ELISA Kit;Human frizzled family receptor 3 ELISA Kit;Human frizzled homolog 3 ELISA Kit;
Catalog
E015588
species
Human
GeneFZD3
Other Species Mouse Fzd3 ELISA Kit Chicken FZD3 ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.469ng/ml
Intended UseHuman FZD3 ELISA Kit allows for the in vitro quantitative determination of FZD3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-FZD3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-FZD3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FZD3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FZD3 can be calculated.
Human Frizzled-3 Protein information
Uniprot ID FZD3_HUMAN
Uniprot AC Q9NPG1; A8K615;
UniGene Hs.40735; Hs.734277;
GeneID 7976
KEGG hsa:7976;
Human Frizzled-3 Protein SEQUENCE
SEQUENCE 666 AA; 76263 MW; 46D7C46E21160215 CRC64;
MAMTWIVFSL WPLTVFMGHI GGHSLFSCEP ITLRMCQDLP YNTTFMPNLL
NHYDQQTAAL AMEPFHPMVN LDCSRDFRPF LCALYAPICM EYGRVTLPCR
RLCQRAYSEC SKLMEMFGVP WPEDMECSRF PDCDEPYPRL VDLNLAGEPT
EGAPVAVQRD YGFWCPRELK IDPDLGYSFL HVRDCSPPCP NMYFRREELS
FARYFIGLIS IICLSATLFT FLTFLIDVTR FRYPERPIIF YAVCYMMVSL
IFFIGFLLED RVACNASIPA QYKASTVTQG SHNKACTMLF MILYFFTMAG
SVWWVILTIT WFLAAVPKWG SEAIEKKALL FHASAWGIPG TLTIILLAMN
KIEGDNISGV CFVGLYDVDA LRYFVLAPLC LYVVVGVSLL LAGIISLNRV
RIEIPLEKEN QDKLVKFMIR IGVFSILYLV PLLVVIGCYF YEQAYRGIWE
TTWIQERCRE YHIPCPYQVT QMSRPDLILF LMKYLMALIV GIPSVFWVGS
KKTCFEWASF FHGRRKKEIV NESRQVLQEP DFAQSLLRDP NTPIIRKSRG
TSTQGTSTHA SSTQLAMVDD QRSKAGSIHS KVSSYHGSLH RSRDGRYTPC
SYRGMEERLP HGSMSRLTDH SRHSSSHRLN EQSRHSSIRD LSNNPMTHIT
HGTSMNRVIE EDGTSA
UCSC uc003xgx.4; human. [Q9NPG1-1];

Category

Human FZD3 ELISA Kit

Human Fz-3 ELISA Kit;Human hFz3 ELISA Kit;Human frizzled class receptor 3 ELISA Kit;Human frizzled-3 ELISA Kit;Human frizzled 3, seven transmembrane spanning receptor ELISA Kit;Human frizzled family receptor 3 ELISA Kit;Human frizzled homolog 3 ELISA Kit;