Human FZD3 ELISA Kit

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  • Alternative name

    Human Fz-3 ELISA Kit;Human hFz3 ELISA Kit;Human frizzled class receptor 3 ELISA Kit;Human frizzled-3 ELISA Kit;Human frizzled 3, seven transmembrane spanning receptor ELISA Kit;Human frizzled family receptor 3 ELISA Kit;Human frizzled homolog 3 ELISA Kit;

  • Catalog
    E015588
  • species
    Human
  • GeneFZD3
  • Other Species Mouse Fzd3 ELISA KitChicken FZD3 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.469ng/ml
  • Intended UseHuman FZD3 ELISA Kit allows for the in vitro quantitative determination of FZD3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-FZD3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-FZD3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FZD3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FZD3 can be calculated.
  • Human Frizzled-3 Protein information
  • Uniprot ID FZD3_HUMAN
  • Uniprot AC Q9NPG1; A8K615;
  • UniGene Hs.40735; Hs.734277;
  • GeneID 7976
  • KEGG hsa:7976;
  • Human Frizzled-3 Protein SEQUENCE
  • SEQUENCE 666 AA; 76263 MW; 46D7C46E21160215 CRC64;

    MAMTWIVFSL WPLTVFMGHI GGHSLFSCEP ITLRMCQDLP YNTTFMPNLL

    NHYDQQTAAL AMEPFHPMVN LDCSRDFRPF LCALYAPICM EYGRVTLPCR

    RLCQRAYSEC SKLMEMFGVP WPEDMECSRF PDCDEPYPRL VDLNLAGEPT

    EGAPVAVQRD YGFWCPRELK IDPDLGYSFL HVRDCSPPCP NMYFRREELS

    FARYFIGLIS IICLSATLFT FLTFLIDVTR FRYPERPIIF YAVCYMMVSL

    IFFIGFLLED RVACNASIPA QYKASTVTQG SHNKACTMLF MILYFFTMAG

    SVWWVILTIT WFLAAVPKWG SEAIEKKALL FHASAWGIPG TLTIILLAMN

    KIEGDNISGV CFVGLYDVDA LRYFVLAPLC LYVVVGVSLL LAGIISLNRV

    RIEIPLEKEN QDKLVKFMIR IGVFSILYLV PLLVVIGCYF YEQAYRGIWE

    TTWIQERCRE YHIPCPYQVT QMSRPDLILF LMKYLMALIV GIPSVFWVGS

    KKTCFEWASF FHGRRKKEIV NESRQVLQEP DFAQSLLRDP NTPIIRKSRG

    TSTQGTSTHA SSTQLAMVDD QRSKAGSIHS KVSSYHGSLH RSRDGRYTPC

    SYRGMEERLP HGSMSRLTDH SRHSSSHRLN EQSRHSSIRD LSNNPMTHIT

    HGTSMNRVIE EDGTSA

  • UCSC uc003xgx.4; human. [Q9NPG1-1];




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