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Human FLOT1 ELISA Kit

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  • Alternative name

    Human flotillin 1 ELISA Kit;Human flotillin-1 ELISA Kit;Human integral membrane component of caveolae ELISA Kit;
  • Catalog
    E015234
  • species
    Human
  • GeneFLOT1
  • Standard CurveHuman FLOT1 ELISA Kit
  • Other Species Mouse Flot1 ELISA KitBovine FLOT1 ELISA KitPorcine FLOT1 ELISA KitRat Flot1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of FLOT1. No significant cross-reactivity or interference between FLOT1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FLOT1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman FLOT1 ELISA Kit allows for the in vitro quantitative determination of FLOT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical    Principle of the Assay: FLOT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FLOT1 antibody and an FLOT1-HRP conjugate. The assay sample and buffer are incubated together with FLOT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FLOT1 concentration since FLOT1 from samples and FLOT1-HRP conjugate compete for the anti-FLOT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by FLOT1 from the sample, fewer sites are left to bind FLOT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FLOT1 concentration in each sample is interpolated from this standard curve.
  • Human Flotillin-1 Protein information
  • Uniprot ID FLOT1_HUMAN
  • Uniprot AC O75955; B4DVY7; Q969J8; Q9UHW1; Q9UNV8;
  • UniGene Hs.179986;
  • GeneID 10211
  • KEGG hsa:10211;
  • Human Flotillin-1 Protein SEQUENCE

  • SEQUENCE 427 AA; 47355 MW; C69E5421E565F53A CRC64;

    MFFTCGPNEA MVVSGFCRSP PVMVAGGRVF VLPCIQQIQR ISLNTLTLNV

    KSEKVYTRHG VPISVTGIAQ VKIQGQNKEM LAAACQMFLG KTEAEIAHIA

    LETLEGHQRA IMAHMTVEEI YKDRQKFSEQ VFKVASSDLV NMGISVVSYT

    LKDIHDDQDY LHSLGKARTA QVQKDARIGE AEAKRDAGIR EAKAKQEKVS

    AQYLSEIEMA KAQRDYELKK AAYDIEVNTR RAQADLAYQL QVAKTKQQIE

    EQRVQVQVVE RAQQVAVQEQ EIARREKELE ARVRKPAEAE RYKLERLAEA

    EKSQLIMQAE AEAASVRMRG EAEAFAIGAR ARAEAEQMAK KAEAFQLYQE

    AAQLDMLLEK LPQVAEEISG PLTSANKITL VSSGSGTMGA AKVTGEVLDI

    LTRLPESVER LTGVSISQVN HKPLRTA



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