Human FASLG ELISA Kit

Alternative name
Human apoptosis antigen ligand ELISA Kit;Human APTL ELISA Kit;Human CD95 ligand ELISA Kit;Human CD95-L ELISA Kit;Human fas antigen ligand ELISA Kit;Human Fas ligand ELISA Kit;Human FASL ELISA Kit;Human Receptor-binding FasL ectodomain ELISA Kit;Human Soluble Fas ligand ELISA Kit;Human sFasL ELISA Kit;Human APL ELISA Kit;Human FasL ICD ELISA Kit;Human SPPL2A-processed FasL form ELISA Kit;Human SPA ELISA Kit;Human APT1LG1 ELISA Kit;Human CD95L ELISA Kit;Human TNFSF6 ELISA Kit;Human ALPS1B ELISA Kit;Human CD178 ELISA Kit;Human TNLG1A ELISA Kit;Human tumor necrosis factor ligand superfamily member 6 ELISA Kit;Human Fas ligand (TNF superfamily, member 6) ELISA Kit;Human apoptosis (APO-1) antigen ligand 1 ELISA Kit;Human mutant tumor necrosis factor family member 6 ELISA Kit;Human tumor necrosis factor ligand 1A ELISA Kit;
Catalog
E014478
species
Human
GeneFASLG
Standard Curve
Other Species Mouse Faslg ELISA Kit Mouse FASL/Faslg ELISA Kit Monkey FASLG ELISA Kit Porcine FASLG ELISA Kit Rat Faslg ELISA Kit
SpecificityNatural and recombinant human FASL
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 2pg/ml
Intended UseHuman FASLG ELISA Kit allows for the in vitro quantitative determination of FASLG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human FASL ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FASL has been precoated onto 96-well plates. Standards (CHO,P134-L281) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FASL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FASL amount of sample captured in plate. Background: Fas ligand (FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.
Human FasL intracellular domain Protein information
Uniprot ID TNFL6_HUMAN
Uniprot AC P48023; Q9BZP9;
UniGene Hs.2007;
GeneID 356
KEGG hsa:356;
Human FasL intracellular domain Protein SEQUENCE
SEQUENCE 281 AA; 31485 MW; A8A6EB358246E9BB CRC64;
MQQPFNYPYP QIYWVDSSAS SPWAPPGTVL PCPTSVPRRP GQRRPPPPPP
PPPLPPPPPP PPLPPLPLPP LKKRGNHSTG LCLLVMFFMV LVALVGLGLG
MFQLFHLQKE LAELRESTSQ MHTASSLEKQ IGHPSPPPEK KELRKVAHLT
GKSNSRSMPL EWEDTYGIVL LSGVKYKKGG LVINETGLYF VYSKVYFRGQ
SCNNLPLSHK VYMRNSKYPQ DLVMMEGKMM SYCTTGQMWA RSSYLGAVFN
LTSADHLYVN VSELSLVNFE ESQTFFGLYK L
UCSC uc001git.4; human. [P48023-1];

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Human FASLG ELISA Kit