Human HMGB2 ELISA Kit

Alternative name
Human high mobility group protein 2 ELISA Kit;Human HMG-2 ELISA Kit;Human HMG2 ELISA Kit;Human high mobility group box 2 ELISA Kit;Human high mobility group protein B2 ELISA Kit;Human high-mobility group (nonhistone chromosomal) protein 2 ELISA Kit;
Catalog
E018631
species
Human
GeneHMGB2
SpecificityThis assay has high sensitivity and excellent specificity for detection of HMGB2. No significant cross-reactivity or interference between HMGB2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HMGB2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman HMGB2 ELISA Kit allows for the in vitro quantitative determination of HMGB2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description
specificalIntended Uses: This HMGB2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HMGB2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: HMGB2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HMGB2 antibody and an HMGB2-HRP conjugate. The assay sample and buffer are incubated together with HMGB2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HMGB2 concentration since HMGB2 from samples and HMGB2-HRP conjugate compete for the anti-HMGB2 antibody binding site. Since the number of sites is limited, as more sites are occupied by HMGB2 from the sample, fewer sites are left to bind HMGB2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMGB2 concentration in each sample is interpolated from this standard curve.
Human High mobility group protein B2 Protein information
Uniprot ID HMGB2_HUMAN
Uniprot AC P26583; B2R4K8; D3DP37; Q5U072;
UniGene Hs.434953;
GeneID 3148
KEGG hsa:3148;
Human High mobility group protein B2 Protein SEQUENCE
SEQUENCE 209 AA; 24034 MW; 5E65292BC4AD8373 CRC64;
MGKGDPNKPR GKMSSYAFFV QTCREEHKKK HPDSSVNFAE FSKKCSERWK
TMSAKEKSKF EDMAKSDKAR YDREMKNYVP PKGDKKGKKK DPNAPKRPPS
AFFLFCSEHR PKIKSEHPGL SIGDTAKKLG EMWSEQSAKD KQPYEQKAAK
LKEKYEKDIA AYRAKGKSEA GKKGPGRPTG SKKKNEPEDE EEEEEEEDED
EEEEDEDEE
UCSC uc003ita.4; human.;

Category

Human HMGB2 ELISA Kit

Human high mobility group protein 2 ELISA Kit;Human HMG-2 ELISA Kit;Human HMG2 ELISA Kit;Human high mobility group box 2 ELISA Kit;Human high mobility group protein B2 ELISA Kit;Human high-mobility group (nonhistone chromosomal) protein 2 ELISA Kit;