Human HMGB2 ELISA Kit

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  • Alternative name

    Human high mobility group protein 2 ELISA Kit;Human HMG-2 ELISA Kit;Human HMG2 ELISA Kit;Human high mobility group box 2 ELISA Kit;Human high mobility group protein B2 ELISA Kit;Human high-mobility group (nonhistone chromosomal) protein 2 ELISA Kit;

  • Catalog
    E018631
  • species
    Human
  • GeneHMGB2
  • Standard CurveHuman HMGB2 ELISA Kit
  • Other Species Mouse Hmgb2 ELISA KitBovine HMGB2 ELISA KitChicken HMGB2 ELISA KitPorcine HMGB2 ELISA KitRat Hmgb2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HMGB2. No significant cross-reactivity or interference between HMGB2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HMGB2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman HMGB2 ELISA Kit allows for the in vitro quantitative determination of HMGB2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Intended Uses: This HMGB2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HMGB2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: HMGB2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HMGB2 antibody and an HMGB2-HRP conjugate. The assay sample and buffer are incubated together with HMGB2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HMGB2 concentration since HMGB2 from samples and HMGB2-HRP conjugate compete for the anti-HMGB2 antibody binding site. Since the number of sites is limited, as more sites are occupied by HMGB2 from the sample, fewer sites are left to bind HMGB2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMGB2 concentration in each sample is interpolated from this standard curve.
  • Human High mobility group protein B2 Protein information
  • Uniprot ID HMGB2_HUMAN
  • Uniprot AC P26583; B2R4K8; D3DP37; Q5U072;
  • UniGene Hs.434953;
  • GeneID 3148
  • KEGG hsa:3148;
  • Human High mobility group protein B2 Protein SEQUENCE
  • SEQUENCE 209 AA; 24034 MW; 5E65292BC4AD8373 CRC64;

    MGKGDPNKPR GKMSSYAFFV QTCREEHKKK HPDSSVNFAE FSKKCSERWK

    TMSAKEKSKF EDMAKSDKAR YDREMKNYVP PKGDKKGKKK DPNAPKRPPS

    AFFLFCSEHR PKIKSEHPGL SIGDTAKKLG EMWSEQSAKD KQPYEQKAAK

    LKEKYEKDIA AYRAKGKSEA GKKGPGRPTG SKKKNEPEDE EEEEEEEDED

    EEEEDEDEE

  • UCSC uc003ita.4; human.;


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