Human EEF1A1 ELISA Kit

Alternative name
Human EF-1-alpha-1 ELISA Kit;Human elongation factor Tu ELISA Kit;Human EF-Tu ELISA Kit;Human eukaryotic elongation factor 1 A-1 ELISA Kit;Human eEF1A-1 ELISA Kit;Human leukocyte receptor cluster member 7 ELISA Kit;Human EEF1A ELISA Kit;Human EF1A ELISA Kit;Human LENG7 ELISA Kit;Human CCS-3 ELISA Kit;Human CCS3 ELISA Kit;Human EE1A1 ELISA Kit;Human EEF-1 ELISA Kit;Human GRAF-1EF ELISA Kit;Human HNGC:16303 ELISA Kit;Human PTI1 ELISA Kit;Human eukaryotic translation elongation factor 1 alpha 1 ELISA Kit;Human elongation factor 1-alpha 1 ELISA Kit;Human CTCL tumor antigen ELISA Kit;Human EF1a-like protein ELISA Kit;Human cervical cancer suppressor 3 ELISA Kit;Human elongation factor 1 alpha subunit ELISA Kit;Human eukaryotic translation elongation factor 1 alpha 1-like 14 ELISA Kit;Human glucocorticoid receptor AF-1 specific elongation factor ELISA Kit;Human leukocyte receptor cluster (LRC) member 7 ELISA Kit;Human prostate tumor-inducing protein 1 ELISA Kit;Human translation elongation factor 1 alpha 1-like 14 ELISA Kit;
Catalog
E013714
species
Human
GeneEEF1A1
Standard Curve
Other Species Mouse Eef1a1 ELISA Kit Bovine EEF1A1 ELISA Kit Hamster EEF1A1 ELISA Kit Horse EEF1A1 ELISA Kit Rabbit EEF1A1 ELISA Kit Rat Eef1a1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1). No significant cross-reactivity or interference between Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.058ng/mL.
Intended UseHuman EEF1A1 ELISA Kit allows for the in vitro quantitative determination of EEF1A1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Elongation factor 1-alpha 1 Protein information
Uniprot ID EF1A1_HUMAN
Uniprot AC P68104; P04719; P04720; Q6IQ15;
UniGene Hs.535192; Hs.586423; Hs.745122;
GeneID 1915
KEGG hsa:1915;
Human Elongation factor 1-alpha 1 Protein SEQUENCE
SEQUENCE 462 AA; 50141 MW; D465615545AF686A CRC64;
MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG
KGSFKYAWVL DKLKAERERG ITIDISLWKF ETSKYYVTII DAPGHRDFIK
NMITGTSQAD CAVLIVAAGV GEFEAGISKN GQTREHALLA YTLGVKQLIV
GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK IGYNPDTVAF VPISGWNGDN
MLEPSANMPW FKGWKVTRKD GNASGTTLLE ALDCILPPTR PTDKPLRLPL
QDVYKIGGIG TVPVGRVETG VLKPGMVVTF APVNVTTEVK SVEMHHEALS
EALPGDNVGF NVKNVSVKDV RRGNVAGDSK NDPPMEAAGF TAQVIILNHP
GQISAGYAPV LDCHTAHIAC KFAELKEKID RRSGKKLEDG PKFLKSGDAA
IVDMVPGKPM CVESFSDYPP LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK
VTKSAQKAQK AK

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Human EEF1A1 ELISA Kit