Human bcl xl ELISA Kit

Contact Us
  • Alternative name

    Bcl-2-like protein 1 ELISA KIT; Apoptosis regulator Bcl-XBCL2L1 ELISA KIT; BCL2L ELISA KIT; BCLX ELISA KIT; Bcl2-L-1 ELISA KIT

  • Catalog
    E004317
  • species
    Human
  • Genebcl xl
  • Standard CurveHuman bcl xl ELISA Kit
  • Other Species Human Bcl-xl ELISA KitMouse Bcl-xl ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman bcl xl ELISA Kit allows for the in vitro quantitative determination of bcl xl , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the Assay: bcl-xl ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-bcl-xl antibody and an bcl-xl-HRP conjugate. The assay sample and buffer are incubated together with bcl-xl-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the bcl-xl concentration since bcl-xl from samples and bcl-xl-HRP conjugate compete for the anti-bcl-xl antibody binding site. Since the number of sites is limited, as more sites are occupied by bcl-xl from the sample, fewer sites are left to bind bcl-xl-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The bcl-xl concentration in each sample is interpolated from this standard curve.


Related products with Human bcl xl ELISA Kit


tag

Inquiry About Human bcl xl ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!