Human ERAlpha ELISA Kit

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  • Alternative name

    Estrogen receptor ELISA KIT; ER-alpha ELISA KIT; Estradiol receptor ELISA KIT; Nuclear receptor subfamily 3 group A member 1ESR1 ELISA KIT; ESR ELISA KIT; NR3A1 ELISA KIT; ER ELISA KIT

  • Catalog
  • species
  • GeneERAlpha
  • Standard CurveHuman ERAlpha ELISA Kit
  • Other Species Mouse ERalpha ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ER?. No significant cross-reactivity or interference between ER? and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ER? and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ERAlpha ELISA Kit allows for the in vitro quantitative determination of ERAlpha , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    Princple of the assay: ER? ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ER? antibody and an ER?-HRP conjugate. The assay sample and buffer are incubated together with ER?-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ER? concentration since ER? from samples and ER?-HRP conjugate compete for the anti-ER? antibody binding site. Since the number of sites is limited, as more sites are occupied by ER? from the sample, fewer sites are left to bind ER?-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ER? concentration in each sample is interpolated from this standard curve.

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