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Alternative name
ERO1-like protein alpha ELISA KIT; Endoplasmic oxidoreductin-1-like protein ELISA KIT; Oxidoreductin-1-L-alphaERO1L ELISA KIT; UNQ434/PRO865 ELISA KIT; ERO1-L ELISA KIT; ERO1-L-alpha ELISA KIT
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Catalog
E013553
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species
Human
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GeneERO1L
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SpecificityThis assay has high sensitivity and excellent specificity for detection of ERO1L. No significant cross-reactivity or interference between ERO1L and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ERO1L and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ng/mL.
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Intended UseHuman ERO1L ELISA Kit allows for the in vitro quantitative determination of ERO1L , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses: This ERO1L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ERO1L. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||ERO1L ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ERO1L antibody and an ERO1L-HRP conjugate. The assay sample and buffer are incubated together with ERO1L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ERO1L concentration since ERO1L from samples and ERO1L-HRP conjugate compete for the anti-ERO1L antibody binding site. Since the number of sites is limited, as more sites are occupied by ERO1L from the sample, fewer sites are left to bind ERO1L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ERO1L concentration in each sample is interpolated from this standard curve.
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