Human GARS ELISA Kit

Alternative name
Human diadenosine tetraphosphate synthetase ELISA Kit;Human AP-4-A synthetase ELISA Kit;Human glycyl-tRNA synthetase ELISA Kit;Human GlyRS ELISA Kit;Human CMT2D ELISA Kit;Human DSMAV ELISA Kit;Human HMN5 ELISA Kit;Human SMAD1 ELISA Kit;Human glycine--tRNA ligase ELISA Kit;Human Charcot-Marie-Tooth neuropathy 2D ELISA Kit;Human Charcot-Marie-Tooth neuropathy, neuronal type, D ELISA Kit;
Catalog
E017198
species
Human
GeneGARS
Standard Curve
Other Species Mouse Gars ELISA Kit Rat Gars ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Glycyl tRNA Synthetase (GARS). No significant cross-reactivity or interference between Glycyl tRNA Synthetase (GARS) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 6.6pg/mL.
Intended UseHuman GARS ELISA Kit allows for the in vitro quantitative determination of GARS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycyl tRNA Synthetase (GARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycyl tRNA Synthetase (GARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycyl tRNA Synthetase (GARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycyl tRNA Synthetase (GARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Glycine--tRNA ligase Protein information
Uniprot ID SYG_HUMAN
Uniprot AC P41250; B3KQA2; B4DIA0; Q969Y1;
UniGene Hs.404321;
GeneID 2617
KEGG hsa:2617;
Human Glycine--tRNA ligase Protein SEQUENCE
SEQUENCE 739 AA; 83166 MW; E4C001CEBF985C59 CRC64;
MPSPRPVLLR GARAALLLLL PPRLLARPSL LLRRSLSAAS CPPISLPAAA
SRSSMDGAGA EEVLAPLRLA VRQQGDLVRK LKEDKAPQVD VDKAVAELKA
RKRVLEAKEL ALQPKDDIVD RAKMEDTLKR RFFYDQAFAI YGGVSGLYDF
GPVGCALKNN IIQTWRQHFI QEEQILEIDC TMLTPEPVLK TSGHVDKFAD
FMVKDVKNGE CFRADHLLKA HLQKLMSDKK CSVEKKSEME SVLAQLDNYG
QQELADLFVN YNVKSPITGN DLSPPVSFNL MFKTFIGPGG NMPGYLRPET
AQGIFLNFKR LLEFNQGKLP FAAAQIGNSF RNEISPRSGL IRVREFTMAE
IEHFVDPSEK DHPKFQNVAD LHLYLYSAKA QVSGQSARKM RLGDAVEQGV
INNTVLGYFI GRIYLYLTKV GISPDKLRFR QHMENEMAHY ACDCWDAESK
TSYGWIEIVG CADRSCYDLS CHARATKVPL VAEKPLKEPK TVNVVQFEPS
KGAIGKAYKK DAKLVMEYLA ICDECYITEM EMLLNEKGEF TIETEGKTFQ
LTKDMINVKR FQKTLYVEEV VPNVIEPSFG LGRIMYTVFE HTFHVREGDE
QRTFFSFPAV VAPFKCSVLP LSQNQEFMPF VKELSEALTR HGVSHKVDDS
SGSIGRRYAR TDEIGVAFGV TIDFDTVNKT PHTATLRDRD SMRQIRAEIS
ELPSIVQDLA NGNITWADVE ARYPLFEGQE TGKKETIEE
UCSC uc003tbm.4; human.;

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Human GARS ELISA Kit