Human AKR1C1 ELISA Kit

Alternative name
Human 20-alpha-hydroxysteroid dehydrogenase ELISA Kit;Human 20-ALPHA-HSD ELISA Kit;Human chlordecone reductase homolog HAKRC ELISA Kit;Human dihydrodiol dehydrogenase 1/2 ELISA Kit;Human DD1/DD2 ELISA Kit;Human high-affinity hepatic bile acid-binding protein ELISA Kit;Human HBAB ELISA Kit;Human indanol dehydrogenase ELISA Kit;Human trans-1,2-dihydrobenzene-1,2-diol dehydrogenase ELISA Kit;Human DDH ELISA Kit;Human DDH1 ELISA Kit;Human 2-ALPHA-HSD ELISA Kit;Human C9 ELISA Kit;Human DD1 ELISA Kit;Human H-37 ELISA Kit;Human HAKRC ELISA Kit;Human MBAB ELISA Kit;Human aldo-keto reductase family 1 member C1 ELISA Kit;Human 20 alpha-hydroxysteroid dehydrogenase ELISA Kit;Human aldo-keto reductase C ELISA Kit;Human dihydrodiol dehydrogenase 1 ELISA Kit;Human dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase ELISA Kit;Human dihydrodiol dehydrogenase isoform DD1 ELISA Kit;Human hepatic dihydrodiol dehydrogenase ELISA Kit;Human type II 3-alpha-hydroxysteroid dehydrogenase ELISA Kit;
Catalog
E001320
species
Human
GeneAKR1C1
Standard Curve
Other Species Mouse AKR1C1 ELISA Kit Monkey AKR1C1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of AKR1C1. No significant cross-reactivity or interference between AKR1C1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AKR1C1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL
Intended UseHuman AKR1C1 ELISA Kit allows for the in vitro quantitative determination of AKR1C1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This AKR1C1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AKR1C1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: AKR1C1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AKR1C1 antibody and an AKR1C1-HRP conjugate. The assay sample and buffer are incubated together with AKR1C1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AKR1C1 concentration since AKR1C1 from samples and AKR1C1-HRP conjugate compete for the anti-AKR1C1 antibody binding site. Since the number of sites is limited, as more sites are occupied by AKR1C1 from the sample, fewer sites are left to bind AKR1C1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AKR1C1 concentration in each sample is interpolated from this standard curve.
Human Aldo-keto reductase family 1 member C1 Protein information
Uniprot ID AK1C1_HUMAN
Uniprot AC Q04828; P52896; Q5SR15; Q7M4N2; Q9UCX2;
UniGene Hs.460260;
GeneID 1645
KEGG hsa:1645;
Human Aldo-keto reductase family 1 member C1 Protein SEQUENCE
SEQUENCE 323 AA; 36788 MW; 9CB215478FBD29D5 CRC64;
MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID
SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE
RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA
VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR
KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR
TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN
RNVRYLTLDI FAGPPNYPFS DEY
UCSC uc001ihq.4; human.;

Category

Human AKR1C1 ELISA Kit