-
Alternative name
Lymphocyte function-associated antigen 3 ELISA KIT; Surface glycoprotein LFA-3 ELISA KIT; CD_antigen: CD58CD58 ELISA KIT; LFA3 ELISA KIT; Ag3 ELISA KIT
-
Catalog
E023154
-
species
Human
-
GeneLFA3
-
SpecificityThis assay has high sensitivity and excellent specificity for detection of Lymphocyte Function Associated Antigen 3 (LFA3).
No significant cross-reactivity or interference between Lymphocyte Function Associated Antigen 3 (LFA3) and analogues was observed.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
SensitivityTypically less than 5.8pg/mL.
-
Intended UseHuman LFA3 ELISA Kit allows for the in vitro quantitative determination of LFA3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Function Associated Antigen 3 (LFA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphocyte Function Associated Antigen 3 (LFA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphocyte Function Associated Antigen 3 (LFA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lymphocyte Function Associated Antigen 3 (LFA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-