Human AKR1B10 ELISA Kit

Alternative name
Human ARL-1 ELISA Kit;Human Aldose reductase-like ELISA Kit;Human aldose reductase-related protein ELISA Kit;Human ARP ELISA Kit;Human hARP ELISA Kit;Human small intestine reductase ELISA Kit;Human SI reductase ELISA Kit;Human AKR1B11 ELISA Kit;Human AKR1B12 ELISA Kit;Human ALDRLn ELISA Kit;Human ARL1 ELISA Kit;Human HIS ELISA Kit;Human HSI ELISA Kit;Human aldo-keto reductase family 1 member B10 ELISA Kit;Human aldo-keto reductase family 1, member B10 (aldose reductase) ELISA Kit;Human aldo-keto reductase family 1, member B11 (aldose reductase-like) ELISA Kit;Human aldose reductase-like 1 ELISA Kit;Human aldose reductase-like peptide ELISA Kit;
Catalog
E001318
species
Human
GeneAKR1B10
Standard Curve
Other Species Mouse AKR1B10 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of AKR1B10. No significant cross-reactivity or interference between AKR1B10 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AKR1B10 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman AKR1B10 ELISA Kit allows for the in vitro quantitative determination of AKR1B10 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: AKR1B10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AKR1B10 antibody and an AKR1B10-HRP conjugate. The assay sample and buffer are incubated together with AKR1B10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AKR1B10 concentration since AKR1B10 from samples and AKR1B10-HRP conjugate compete for the anti-AKR1B10 antibody binding site. Since the number of sites is limited, as more sites are occupied by AKR1B10 from the sample, fewer sites are left to bind AKR1B10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AKR1B10 concentration in each sample is interpolated from this standard curve.
Human Aldo-keto reductase family 1 member B10 Protein information
Uniprot ID AK1BA_HUMAN
Uniprot AC O60218; A4D1P1; O75890; Q6FHF3; Q8IWZ1;
UniGene Hs.116724;
GeneID 57016
KEGG hsa:57016;
Human Aldo-keto reductase family 1 member B10 Protein SEQUENCE
SEQUENCE 316 AA; 36020 MW; 0C2FC0E798955A33 CRC64;
MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ
NEHEVGEAIQ EKIQEKAVKR EDLFIVSKLW PTFFERPLVR KAFEKTLKDL
KLSYLDVYLI HWPQGFKSGD DLFPKDDKGN AIGGKATFLD AWEAMEELVD
EGLVKALGVS NFSHFQIEKL LNKPGLKYKP VTNQVECHPY LTQEKLIQYC
HSKGITVTAY SPLGSPDRPW AKPEDPSLLE DPKIKEIAAK HKKTAAQVLI
RFHIQRNVIV IPKSVTPARI VENIQVFDFK LSDEEMATIL SFNRNWRACN
VLQSSHLEDY PFNAEY
UCSC uc003vrr.4; human.;

Category

Human AKR1B10 ELISA Kit