Human EDIL3 ELISA Kit

Alternative name
Human developmentally-regulated endothelial cell locus 1 protein ELISA Kit;Human integrin-binding protein DEL1 ELISA Kit;Human DEL1 ELISA Kit;Human EGF like repeats and discoidin domains 3 ELISA Kit;Human EGF-like repeat and discoidin I-like domain-containing protein 3 ELISA Kit;Human EGF-like repeats and discoidin I-like domains 3 ELISA Kit;Human developmental endothelial locus-1 ELISA Kit;
Catalog
E012765
species
Human
GeneEDIL3
SpecificityNatural and recombinant Human EDIL3
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Intra-assay Precision Intra-Assay Precision (Precision within an assay) Three samples of kn
Intended UseHuman EDIL3 ELISA Kit allows for the in vitro quantitative determination of EDIL3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: Human EDIL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EDIL3 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: D24-E480) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EDIL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin- Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EDIL3 amount of sample captured in plate. Background/Introduction: EDIL3, also known as DEL1, is a glycoprotein that is secreted by endothelial cells and can associate with the endothelial cell surface and the extracellular matrix. It is regulated by hypoxia or vascular injury and has been implicated in vascular remodeling during angiogenesis. The EDIL3 gene is mapped to 5q14. Human endothelial cells bind to Del1 together with the alpha-V (ITGAV)/beta-3 (ITGB3) integrin receptor. It is confirmed that adhesion of endothelial cells to Del1 is integrin mediated and that Del1 binds ITGAV/ITGB3. Functional analysis showed that both Del1 and vitronectin (VTN) stimulate endothelial cell migration. Immunoprecipitation and immunoblot analyses revealed that adhesion of endothelial cells to Del1 induces tyrosine phosphorylation of PTK2.
Human EGF-like repeat and discoidin I-like domain-containing protein 3 Protein information
Uniprot ID EDIL3_HUMAN
Uniprot AC O43854; B2R763; O43855; Q5D094; Q8N610;
UniGene Hs.482730;
GeneID 10085
KEGG hsa:10085;
Human EGF-like repeat and discoidin I-like domain-containing protein 3 Protein SEQUENCE
SEQUENCE 480 AA; 53765 MW; F7171E23A309FD48 CRC64;
MKRSVAVWLL VGLSLGVPQF GKGDICDPNP CENGGICLPG LADGSFSCEC
PDGFTDPNCS SVVEVASDEE EPTSAGPCTP NPCHNGGTCE ISEAYRGDTF
IGYVCKCPRG FNGIHCQHNI NECEVEPCKN GGICTDLVAN YSCECPGEFM
GRNCQYKCSG PLGIEGGIIS NQQITASSTH RALFGLQKWY PYYARLNKKG
LINAWTAAEN DRWPWIQINL QRKMRVTGVI TQGAKRIGSP EYIKSYKIAY
SNDGKTWAMY KVKGTNEDMV FRGNIDNNTP YANSFTPPIK AQYVRLYPQV
CRRHCTLRME LLGCELSGCS EPLGMKSGHI QDYQITASSI FRTLNMDMFT
WEPRKARLDK QGKVNAWTSG HNDQSQWLQV DLLVPTKVTG IITQGAKDFG
HVQFVGSYKL AYSNDGEHWT VYQDEKQRKD KVFQGNFDND THRKNVIDPP
IYARHIRILP WSWYGRITLR SELLGCTEEE
UCSC uc003kio.3; human. [O43854-1];

Category

Human EDIL3 ELISA Kit