Human PDS5B ELISA Kit

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  • Alternative name

    Human androgen-induced proliferation inhibitor ELISA Kit;Human androgen-induced prostate proliferative shutoff-associated protein AS3 ELISA Kit;Human APRIN ELISA Kit;Human AS3 ELISA Kit;Human CG008 ELISA Kit;Human PDS5 cohesin associated factor B ELISA Kit;Human sister chromatid cohesion protein PDS5 homolog B ELISA Kit;Human androgen induced inhibitor of proliferation ELISA Kit;Human androgen-induced shutoff 3 ELISA Kit;

  • Catalog
    E035364
  • species
    Human
  • GenePDS5B
  • Standard CurveHuman PDS5B ELISA Kit
  • Other Species Mouse Pds5b ELISA KitChicken PDS5B ELISA KitRat Pds5b ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PDS5B. No significant cross-reactivity or interference between PDS5B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PDS5B and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PDS5B ELISA Kit allows for the in vitro quantitative determination of PDS5B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Intended Uses||This PDS5B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PDS5B. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: PDS5B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PDS5B antibody and an PDS5B-HRP conjugate. The assay sample and buffer are incubated together with PDS5B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PDS5B concentration since PDS5B from samples and PDS5B-HRP conjugate compete for the anti-PDS5B antibody binding site. Since the number of sites is limited, as more sites are occupied by PDS5B from the sample, fewer sites are left to bind PDS5B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDS5B concentration in each sample is interpolated from this standard curve.
  • Human Sister chromatid cohesion protein PDS5 homolog B Protein information
  • Uniprot ID PDS5B_HUMAN
  • Uniprot AC Q9NTI5; Q5R3S3; Q5W0K8; Q6NSC3; Q8IXT6; Q9H5N8; Q9Y2I5; Q9Y451;
  • UniGene Hs.744901;
  • GeneID 23047
  • KEGG hsa:23047;
  • Human Sister chromatid cohesion protein PDS5 homolog B Protein SEQUENCE
  • SEQUENCE 1447 AA; 164667 MW; 145C30308EA3EFD5 CRC64;

    MAHSKTRTND GKITYPPGVK EISDKISKEE MVRRLKMVVK TFMDMDQDSE

    EEKELYLNLA LHLASDFFLK HPDKDVRLLV ACCLADIFRI YAPEAPYTSP

    DKLKDIFMFI TRQLKGLEDT KSPQFNRYFY LLENIAWVKS YNICFELEDS

    NEIFTQLYRT LFSVINNGHN QKVHMHMVDL MSSIICEGDT VSQELLDTVL

    VNLVPAHKNL NKQAYDLAKA LLKRTAQAIE PYITNFFNQV LMLGKTSISD

    LSEHVFDLIL ELYNIDSHLL LSVLPQLEFK LKSNDNEERL QVVKLLAKMF

    GAKDSELASQ NKPLWQCYLG RFNDIHVPIR LECVKFASHC LMNHPDLAKD

    LTEYLKVRSH DPEEAIRHDV IVSIVTAAKK DILLVNDHLL NFVRERTLDK

    RWRVRKEAMM GLAQIYKKYA LQSAAGKDAA KQIAWIKDKL LHIYYQNSID

    DRLLVERIFA QYMVPHNLET TERMKCLYYL YATLDLNAVK ALNEMWKCQN

    LLRHQVKDLL DLIKQPKTDA SVKAIFSKVM VITRNLPDPG KAQDFMKKFT

    QVLEDDEKIR KQLEVLVSPT CSCKQAEGCV REITKKLGNP KQPTNPFLEM

    IKFLLERIAP VHIDTESISA LIKQVNKSID GTADDEDEGV PTDQAIRAGL

    ELLKVLSFTH PISFHSAETF ESLLACLKMD DEKVAEAALQ IFKNTGSKIE

    EDFPHIRSAL LPVLHHKSKK GPPRQAKYAI HCIHAIFSSK ETQFAQIFEP

    LHKSLDPSNL EHLITPLVTI GHIALLAPDQ FAAPLKSLVA TFIVKDLLMN

    DRLPGKKTTK LWVPDEEVSP ETMVKIQAIK MMVRWLLGMK NNHSKSGTST

    LRLLTTILHS DGDLTEQGKI SKPDMSRLRL AAGSAIVKLA QEPCYHEIIT

    LEQYQLCALA INDECYQVRQ VFAQKLHKGL SRLRLPLEYM AICALCAKDP

    VKERRAHARQ CLVKNINVRR EYLKQHAAVS EKLLSLLPEY VVPYTIHLLA

    HDPDYVKVQD IEQLKDVKEC LWFVLEILMA KNENNSHAFI RKMVENIKQT

    KDAQGPDDAK MNEKLYTVCD VAMNIIMSKS TTYSLESPKD PVLPARFFTQ

    PDKNFSNTKN YLPPEMKSFF TPGKPKTTNV LGAVNKPLSS AGKQSQTKSS

    RMETVSNASS SSNPSSPGRI KGRLDSSEMD HSENEDYTMS SPLPGKKSDK

    RDDSDLVRSE LEKPRGRKKT PVTEQEEKLG MDDLTKLVQE QKPKGSQRSR

    KRGHTASESD EQQWPEEKRL KEDILENEDE QNSPPKKGKR GRPPKPLGGG

    TPKEEPTMKT SKKGSKKKSG PPAPEEEEEE ERQSGNTEQK SKSKQHRVSR

    RAQQRAESPE SSAIESTQST PQKGRGRPSK TPSPSQPKKN VRVGRSKQAA

    TKENDSSEEV DVFQGSSPVD DIPQEETEEE EVSTVNVRRR SAKRERR

  • UCSC uc010abf.4; human. [Q9NTI5-1];


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