Human ADH4 ELISA Kit

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  • Alternative name

    Human alcohol dehydrogenase class II pi chain ELISA Kit;Human ADH-2 ELISA Kit;Human HEL-S-4 ELISA Kit;Human alcohol dehydrogenase 4 (class II), pi polypeptide ELISA Kit;Human alcohol dehydrogenase 4 ELISA Kit;Human aldehyde reductase ELISA Kit;Human epididymis secretory protein Li 4 ELISA Kit;

  • Catalog
    E001266
  • species
    Human
  • GeneADH4
  • Standard CurveHuman ADH4 ELISA Kit
  • Other Species Mouse Adh4 ELISA KitRat Adh4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ADH4. No significant cross-reactivity or interference between ADH4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADH4 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman ADH4 ELISA Kit allows for the in vitro quantitative determination of ADH4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: This ADH4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADH4. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ADH4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ADH4 antibody and an ADH4-HRP conjugate. The assay sample and buffer are incubated together with ADH4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADH4 concentration since ADH4 from samples and ADH4-HRP conjugate compete for the anti-ADH4 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADH4 from the sample, fewer sites are left to bind ADH4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADH4 concentration in each sample is interpolated from this standard curve.
  • Human Alcohol dehydrogenase 4 Protein information
  • Uniprot ID ADH4_HUMAN
  • Uniprot AC P08319; A8K470; B4DIE7; C9J4A9; Q8TCD7;
  • UniGene Hs.1219;
  • GeneID 127
  • KEGG hsa:127;
  • Human Alcohol dehydrogenase 4 Protein SEQUENCE
  • SEQUENCE 380 AA; 40222 MW; 45721A08197629F1 CRC64;

    MGTKGKVIKC KAAIAWEAGK PLCIEEVEVA PPKAHEVRIQ IIATSLCHTD

    ATVIDSKFEG LAFPVIVGHE AAGIVESIGP GVTNVKPGDK VIPLYAPLCR

    KCKFCLSPLT NLCGKISNLK SPASDQQLME DKTSRFTCKG KPVYHFFGTS

    TFSQYTVVSD INLAKIDDDA NLERVCLLGC GFSTGYGAAI NNAKVTPGST

    CAVFGLGGVG LSAVMGCKAA GASRIIGIDI NSEKFVKAKA LGATDCLNPR

    DLHKPIQEVI IELTKGGVDF ALDCAGGSET MKAALDCTTA GWGSCTFIGV

    AAGSKGLTIF PEELIIGRTI NGTFFGGWKS VDSIPKLVTD YKNKKFNLDA

    LVTHTLPFDK ISEAFDLMNQ GKSVRTILIF

  • UCSC uc003hun.4; human. [P08319-1];


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