Human DMGDH ELISA Kit

Alternative name
Human ME2GLYDH ELISA Kit;Human DMGDHD ELISA Kit;Human dimethylglycine dehydrogenase ELISA Kit;Human dimethylglycine dehydrogenase, mitochondrial ELISA Kit;
Catalog
E011979
species
Human
GeneDMGDH
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<46.9pg/ml
Intended UseHuman DMGDH ELISA Kit allows for the in vitro quantitative determination of DMGDH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-DMGDH antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-DMGDH antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the DMGDH amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of DMGDH can be calculated.
Human Dimethylglycine dehydrogenase, mitochondrial Protein information
Uniprot ID M2GD_HUMAN
Uniprot AC Q9UI17; B2RBN0; B4E1J9;
UniGene Hs.655653;
GeneID 29958
KEGG hsa:29958;
Human Dimethylglycine dehydrogenase, mitochondrial Protein SEQUENCE
SEQUENCE 866 AA; 96811 MW; 2EC1FA7DCB6C1F8C CRC64;
MLRPGAQLLR GLLLRSCPLQ GSPGRPRSVC GREGEEKPPL SAETQWKDRA
ETVIIGGGCV GVSLAYHLAK AGMKDVVLLE KSELTAGSTW HAAGLTTYFH
PGINLKKIHY DSIKLYEKLE EETGQVVGFH QPGSIRLATT PVRVDEFKYQ
MTRTGWHATE QYLIEPEKIQ EMFPLLNMNK VLAGLYNPGD GHIDPYSLTM
ALAAGARKCG ALLKYPAPVT SLKARSDGTW DVETPQGSMR ANRIVNAAGF
WAREVGKMIG LEHPLIPVQH QYVVTSTISE VKALKRELPV LRDLEGSYYL
RQERDGLLFG PYESQEKMKV QDSWVTNGVP PGFGKELFES DLDRIMEHIK
AAMEMVPVLK KADIINVVNG PITYSPDILP MVGPHQGVRN YWVAIGFGYG
IIHAGGVGKY LSDWILHGEP PFDLIELDPN RYGKWTTTQY TEAKARESYG
FNNIVGYPKE ERFAGRPTQR VSGLYQRLES KCSMGFHAGW EQPHWFYKPG
QDTQYRPSFR RTNWFEPVGS EYKQVMQRVA VTDLSPFGKF NIKGQDSIRL
LDHLFANVIP KVGFTNISHM LTPKGRVYAE LTVSHQSPGE FLLITGSGSE
LHDLRWIEEE AVKGGYDVEI KNITDELGVL GVAGPQARKV LQKLTSEDLS
DDVFKFLQTK SLKVSNIPVT AIRISYTGEL GWELYHRRED SVALYDAIMN
AGQEEGIDNF GTYAMNALRL EKAFRAWGLE MNCDTNPLEA GLEYFVKLNK
PADFIGKQAL KQIKAKGLKR RLVCLTLATD DVDPEGNESI WYNGKVVGNT
TSGSYSYSIQ KSLAFAYVPV QLSEVGQQVE VELLGKNYPA VIIQEPLVLT
EPTRNRLQKK GGKDKT
UCSC uc003kfs.5; human. [Q9UI17-1];

Category

Human DMGDH ELISA Kit

Human ME2GLYDH ELISA Kit;Human DMGDHD ELISA Kit;Human dimethylglycine dehydrogenase ELISA Kit;Human dimethylglycine dehydrogenase, mitochondrial ELISA Kit;