SpecificityThis assay has high sensitivity and excellent specificity for detection of human beta-defensins 1. No significant cross-reactivity or interference between human beta-defensins 1 and analogues was observed.
SensitivityThe minimum detectable dose of human beta-defensins is typically less than 0.78 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determine
Intended UseHuman DEFB1 ELISA Kit allows for the in vitro quantitative determination of DEFB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for beta-defensins 1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any beta-defensins 1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for beta-defensins 1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of beta-defensins 1 bound in the initial step. The color development is stopped and the intensity of the color is measured.