Human ACAN ELISA Kit

Alternative name
Human Cartilage-specific proteoglycan core protein ELISA Kit;Human CSPCP ELISA Kit;Human Chondroitin sulfate proteoglycan core protein 1 ELISA Kit;Human Chondroitin sulfate proteoglycan 1 ELISA Kit;Human AGC1 ELISA Kit;Human CSPG1 ELISA Kit;Human MSK16 ELISA Kit;
Catalog
E001168
species
Human
GeneACAN
Standard Curve
Other Species Mouse Acan ELISA Kit Bovine ACAN ELISA Kit Canine ACAN ELISA Kit Chicken ACAN ELISA Kit Porcine ACAN ELISA Kit Rabbit ACAN ELISA Kit Rat Acan ELISA Kit
SpecificityNatural and recombinant human Aggrecan
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Sample For quantitative detection of human Aggrecan in cell culture supernates, serum and u
Intended UseHuman ACAN ELISA Kit allows for the in vitro quantitative determination of ACAN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: Human Aggrecan ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Aggrecan has been precoated onto 96-well plates. Standards (NSO, V20-G675) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Aggrecan is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Aggrecan amount of sample captured in plate. Background: Aggrecan, also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. It is a critical component for cartilage structure and the function of joints. Aggrecan provides intervertebral disc and cartilage with the ability to resist compressive loads. The localized high concentrations of aggrecan provide the osmotic properties necessary for normal tissue function with the GAGs producing the swelling pressure that counters compressive loads on the tissue. This functional ability is dependent on a high GAG/aggrecan concentration being present in the tissue extracellular matrix. Also, Aggrecan plays an important role in the organization of the extracellular spaces between neurons in the brain.
Human Aggrecan core protein 2 Protein information
Uniprot ID PGCA_HUMAN
Uniprot AC P16112; Q13650; Q9UCD3; Q9UCP4; Q9UCP5; Q9UDE0;
UniGene Hs.2159; Hs.616395;
Human Aggrecan core protein 2 Protein SEQUENCE
SEQUENCE 2415 AA; 250193 MW; 1288937E1B98C6B6 CRC64;
MTTLLWVFVT LRVITAAVTV ETSDHDNSLS VSIPQPSPLR VLLGTSLTIP
CYFIDPMHPV TTAPSTAPLA PRIKWSRVSK EKEVVLLVAT EGRVRVNSAY
QDKVSLPNYP AIPSDATLEV QSLRSNDSGV YRCEVMHGIE DSEATLEVVV
KGIVFHYRAI STRYTLDFDR AQRACLQNSA IIATPEQLQA AYEDGFHQCD
AGWLADQTVR YPIHTPREGC YGDKDEFPGV RTYGIRDTNE TYDVYCFAEE
MEGEVFYATS PEKFTFQEAA NECRRLGARL ATTGHVYLAW QAGMDMCSAG
WLADRSVRYP ISKARPNCGG NLLGVRTVYV HANQTGYPDP SSRYDAICYT
GEDFVDIPEN FFGVGGEEDI TVQTVTWPDM ELPLPRNITE GEARGSVILT
VKPIFEVSPS PLEPEEPFTF APEIGATAFA EVENETGEAT RPWGFPTPGL
GPATAFTSED LVVQVTAVPG QPHLPGGVVF HYRPGPTRYS LTFEEAQQAC
PGTGAVIASP EQLQAAYEAG YEQCDAGWLR DQTVRYPIVS PRTPCVGDKD
SSPGVRTYGV RPSTETYDVY CFVDRLEGEV FFATRLEQFT FQEALEFCES
HNATATTGQL YAAWSRGLDK CYAGWLADGS LRYPIVTPRP ACGGDKPGVR
TVYLYPNQTG LPDPLSRHHA FCFRGISAVP SPGEEEGGTP TSPSGVEEWI
VTQVVPGVAA VPVEEETTAV PSGETTAILE FTTEPENQTE WEPAYTPVGT
SPLPGILPTW PPTGAETEES TEGPSATEVP SASEEPSPSE VPFPSEEPSP
SEEPFPSVRP FPSVELFPSE EPFPSKEPSP SEEPSASEEP YTPSPPEPSW
TELPSSGEES GAPDVSGDFT GSGDVSGHLD FSGQLSGDRA SGLPSGDLDS
SGLTSTVGSG LTVESGLPSG DEERIEWPST PTVGELPSGA EILEGSASGV
GDLSGLPSGE VLETSASGVG DLSGLPSGEV LETTAPGVED ISGLPSGEVL
ETTAPGVEDI SGLPSGEVLE TTAPGVEDIS GLPSGEVLET TAPGVEDISG
LPSGEVLETT APGVEDISGL PSGEVLETAA PGVEDISGLP SGEVLETAAP
GVEDISGLPS GEVLETAAPG VEDISGLPSG EVLETAAPGV EDISGLPSGE
VLETAAPGVE DISGLPSGEV LETAAPGVED ISGLPSGEVL ETAAPGVEDI
SGLPSGEVLE TAAPGVEDIS GLPSGEVLET AAPGVEDISG LPSGEVLETA
APGVEDISGL PSGEVLETAA PGVEDISGLP SGEVLETTAP GVEEISGLPS
GEVLETTAPG VDEISGLPSG EVLETTAPGV EEISGLPSGE VLETSTSAVG
DLSGLPSGGE VLEISVSGVE DISGLPSGEV VETSASGIED VSELPSGEGL
ETSASGVEDL SRLPSGEEVL EISASGFGDL SGVPSGGEGL ETSASEVGTD
LSGLPSGREG LETSASGAED LSGLPSGKED LVGSASGDLD LGKLPSGTLG
SGQAPETSGL PSGFSGEYSG VDLGSGPPSG LPDFSGLPSG FPTVSLVDST
LVEVVTASTA SELEGRGTIG ISGAGEISGL PSSELDISGR ASGLPSGTEL
SGQASGSPDV SGEIPGLFGV SGQPSGFPDT SGETSGVTEL SGLSSGQPGV
SGEASGVLYG TSQPFGITDL SGETSGVPDL SGQPSGLPGF SGATSGVPDL
VSGTTSGSGE SSGITFVDTS LVEVAPTTFK EEEGLGSVEL SGLPSGEADL
SGKSGMVDVS GQFSGTVDSS GFTSQTPEFS GLPSGIAEVS GESSRAEIGS
SLPSGAYYGS GTPSSFPTVS LVDRTLVESV TQAPTAQEAG EGPSGILELS
GAHSGAPDMS GEHSGFLDLS GLQSGLIEPS GEPPGTPYFS GDFASTTNVS
GESSVAMGTS GEASGLPEVT LITSEFVEGV TEPTISQELG QRPPVTHTPQ
LFESSGKVST AGDISGATPV LPGSGVEVSS VPESSSETSA YPEAGFGASA
APEASREDSG SPDLSETTSA FHEANLERSS GLGVSGSTLT FQEGEASAAP
EVSGESTTTS DVGTEAPGLP SATPTASGDR TEISGDLSGH TSQLGVVIST
SIPESEWTQQ TQRPAETHLE IESSSLLYSG EETHTVETAT SPTDASIPAS
PEWKRESEST AAAPARSCAE EPCGAGTCKE TEGHVICLCP PGYTGEHCNI
DQEVCEEGWN KYQGHCYRHF PDRETWVDAE RRCREQQSHL SSIVTPEEQE
FVNNNAQDYQ WIGLNDRTIE GDFRWSDGHP MQFENWRPNQ PDNFFAAGED
CVVMIWHEKG EWNDVPCNYH LPFTCKKGTV ACGEPPVVEH ARTFGQKKDR
YEINSLVRYQ CTEGFVQRHM PTIRCQPSGH WEEPRITCTD ATTYKRRLQK
RSSRHPRRSR PSTAH

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Human ACAN ELISA Kit

Human Cartilage-specific proteoglycan core protein ELISA Kit;Human CSPCP ELISA Kit;Human Chondroitin sulfate proteoglycan core protein 1 ELISA Kit;Human Chondroitin sulfate proteoglycan 1 ELISA Kit;Human AGC1 ELISA Kit;Human CSPG1 ELISA Kit;Human MSK16 ELISA Kit;