Human ACAN ELISA Kit

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  • Alternative name

    Human Cartilage-specific proteoglycan core protein ELISA Kit;Human CSPCP ELISA Kit;Human Chondroitin sulfate proteoglycan core protein 1 ELISA Kit;Human Chondroitin sulfate proteoglycan 1 ELISA Kit;Human AGC1 ELISA Kit;Human CSPG1 ELISA Kit;Human MSK16 ELISA Kit;

  • Catalog
    E001168
  • species
    Human
  • GeneACAN
  • Standard CurveHuman ACAN ELISA Kit
  • Other Species Mouse Acan ELISA KitBovine ACAN ELISA KitCanine ACAN ELISA KitChicken ACAN ELISA KitPorcine ACAN ELISA KitRabbit ACAN ELISA KitRat Acan ELISA Kit
  • SpecificityNatural and recombinant human Aggrecan
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Sample For quantitative detection of human Aggrecan in cell culture supernates, serum and u
  • Intended UseHuman ACAN ELISA Kit allows for the in vitro quantitative determination of ACAN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: Human Aggrecan ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Aggrecan has been precoated onto 96-well plates. Standards (NSO, V20-G675) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Aggrecan is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Aggrecan amount of sample captured in plate. Background: Aggrecan, also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. It is a critical component for cartilage structure and the function of joints. Aggrecan provides intervertebral disc and cartilage with the ability to resist compressive loads. The localized high concentrations of aggrecan provide the osmotic properties necessary for normal tissue function with the GAGs producing the swelling pressure that counters compressive loads on the tissue. This functional ability is dependent on a high GAG/aggrecan concentration being present in the tissue extracellular matrix. Also, Aggrecan plays an important role in the organization of the extracellular spaces between neurons in the brain.
  • Human Aggrecan core protein 2 Protein information
  • Uniprot ID PGCA_HUMAN
  • Uniprot AC P16112; Q13650; Q9UCD3; Q9UCP4; Q9UCP5; Q9UDE0;
  • UniGene Hs.2159; Hs.616395;
  • Human Aggrecan core protein 2 Protein SEQUENCE
  • SEQUENCE 2415 AA; 250193 MW; 1288937E1B98C6B6 CRC64;

    MTTLLWVFVT LRVITAAVTV ETSDHDNSLS VSIPQPSPLR VLLGTSLTIP

    CYFIDPMHPV TTAPSTAPLA PRIKWSRVSK EKEVVLLVAT EGRVRVNSAY

    QDKVSLPNYP AIPSDATLEV QSLRSNDSGV YRCEVMHGIE DSEATLEVVV

    KGIVFHYRAI STRYTLDFDR AQRACLQNSA IIATPEQLQA AYEDGFHQCD

    AGWLADQTVR YPIHTPREGC YGDKDEFPGV RTYGIRDTNE TYDVYCFAEE

    MEGEVFYATS PEKFTFQEAA NECRRLGARL ATTGHVYLAW QAGMDMCSAG

    WLADRSVRYP ISKARPNCGG NLLGVRTVYV HANQTGYPDP SSRYDAICYT

    GEDFVDIPEN FFGVGGEEDI TVQTVTWPDM ELPLPRNITE GEARGSVILT

    VKPIFEVSPS PLEPEEPFTF APEIGATAFA EVENETGEAT RPWGFPTPGL

    GPATAFTSED LVVQVTAVPG QPHLPGGVVF HYRPGPTRYS LTFEEAQQAC

    PGTGAVIASP EQLQAAYEAG YEQCDAGWLR DQTVRYPIVS PRTPCVGDKD

    SSPGVRTYGV RPSTETYDVY CFVDRLEGEV FFATRLEQFT FQEALEFCES

    HNATATTGQL YAAWSRGLDK CYAGWLADGS LRYPIVTPRP ACGGDKPGVR

    TVYLYPNQTG LPDPLSRHHA FCFRGISAVP SPGEEEGGTP TSPSGVEEWI

    VTQVVPGVAA VPVEEETTAV PSGETTAILE FTTEPENQTE WEPAYTPVGT

    SPLPGILPTW PPTGAETEES TEGPSATEVP SASEEPSPSE VPFPSEEPSP

    SEEPFPSVRP FPSVELFPSE EPFPSKEPSP SEEPSASEEP YTPSPPEPSW

    TELPSSGEES GAPDVSGDFT GSGDVSGHLD FSGQLSGDRA SGLPSGDLDS

    SGLTSTVGSG LTVESGLPSG DEERIEWPST PTVGELPSGA EILEGSASGV

    GDLSGLPSGE VLETSASGVG DLSGLPSGEV LETTAPGVED ISGLPSGEVL

    ETTAPGVEDI SGLPSGEVLE TTAPGVEDIS GLPSGEVLET TAPGVEDISG

    LPSGEVLETT APGVEDISGL PSGEVLETAA PGVEDISGLP SGEVLETAAP

    GVEDISGLPS GEVLETAAPG VEDISGLPSG EVLETAAPGV EDISGLPSGE

    VLETAAPGVE DISGLPSGEV LETAAPGVED ISGLPSGEVL ETAAPGVEDI

    SGLPSGEVLE TAAPGVEDIS GLPSGEVLET AAPGVEDISG LPSGEVLETA

    APGVEDISGL PSGEVLETAA PGVEDISGLP SGEVLETTAP GVEEISGLPS

    GEVLETTAPG VDEISGLPSG EVLETTAPGV EEISGLPSGE VLETSTSAVG

    DLSGLPSGGE VLEISVSGVE DISGLPSGEV VETSASGIED VSELPSGEGL

    ETSASGVEDL SRLPSGEEVL EISASGFGDL SGVPSGGEGL ETSASEVGTD

    LSGLPSGREG LETSASGAED LSGLPSGKED LVGSASGDLD LGKLPSGTLG

    SGQAPETSGL PSGFSGEYSG VDLGSGPPSG LPDFSGLPSG FPTVSLVDST

    LVEVVTASTA SELEGRGTIG ISGAGEISGL PSSELDISGR ASGLPSGTEL

    SGQASGSPDV SGEIPGLFGV SGQPSGFPDT SGETSGVTEL SGLSSGQPGV

    SGEASGVLYG TSQPFGITDL SGETSGVPDL SGQPSGLPGF SGATSGVPDL

    VSGTTSGSGE SSGITFVDTS LVEVAPTTFK EEEGLGSVEL SGLPSGEADL

    SGKSGMVDVS GQFSGTVDSS GFTSQTPEFS GLPSGIAEVS GESSRAEIGS

    SLPSGAYYGS GTPSSFPTVS LVDRTLVESV TQAPTAQEAG EGPSGILELS

    GAHSGAPDMS GEHSGFLDLS GLQSGLIEPS GEPPGTPYFS GDFASTTNVS

    GESSVAMGTS GEASGLPEVT LITSEFVEGV TEPTISQELG QRPPVTHTPQ

    LFESSGKVST AGDISGATPV LPGSGVEVSS VPESSSETSA YPEAGFGASA

    APEASREDSG SPDLSETTSA FHEANLERSS GLGVSGSTLT FQEGEASAAP

    EVSGESTTTS DVGTEAPGLP SATPTASGDR TEISGDLSGH TSQLGVVIST

    SIPESEWTQQ TQRPAETHLE IESSSLLYSG EETHTVETAT SPTDASIPAS

    PEWKRESEST AAAPARSCAE EPCGAGTCKE TEGHVICLCP PGYTGEHCNI

    DQEVCEEGWN KYQGHCYRHF PDRETWVDAE RRCREQQSHL SSIVTPEEQE

    FVNNNAQDYQ WIGLNDRTIE GDFRWSDGHP MQFENWRPNQ PDNFFAAGED

    CVVMIWHEKG EWNDVPCNYH LPFTCKKGTV ACGEPPVVEH ARTFGQKKDR

    YEINSLVRYQ CTEGFVQRHM PTIRCQPSGH WEEPRITCTD ATTYKRRLQK

    RSSRHPRRSR PSTAH



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