Human AFP ELISA Kit

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  • Alternative name

    Human alpha-1-fetoprotein ELISA Kit;Human alpha-fetoglobulin ELISA Kit;Human HPAFP ELISA Kit;Human AFPD ELISA Kit;Human FETA ELISA Kit;Human alpha fetoprotein ELISA Kit;Human alpha-fetoprotein ELISA Kit;

  • Catalog
    E001147
  • species
    Human
  • GeneAFP
  • Standard CurveHuman AFP ELISA Kit
  • Other Species Human AFP-L3 ELISA KitHuman AFP L1 ELISA KitHuman AFP L2 ELISA KitHuman AFP-L2 ELISA KitHuman AFP.L3 ELISA KitHuman AFP-L1 ELISA KitMouse Afp ELISA KitNude Mouse AFP ELISA KitMouse AFP-L2 ELISA KitMouse AFP-L1 ELISA KitMouse AFP-L3 ELISA KitBovine AFP ELISA KitCanine AFP ELISA KitChicken AFP ELISA KitHorse AFP ELISA KitPorcine AFP ELISA KitRat Afp ELISA Kit
  • SpecificityNatural and recombinant human AFP
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Sample For quantitative detection of human AFP in cell culture supernates, serum and plasma
  • Intended UseHuman AFP ELISA Kit allows for the in vitro quantitative determination of AFP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: Human AFP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for AFP has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: R19-V609) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for AFP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human AFP amount of sample captured in plate. Background: Alpha-fetoprotein (AFP, alpha-fetoprotein; also sometimes called alpha-1-fetoprotein, alpha-fetoglobulin, or alpha fetal protein) is a protein that in humans is encoded by the AFP gene. The AFP gene is located on the q arm of chromosome 4 (4q25). AFP is the most abundant plasma protein found in the human fetus. Plasma levels decrease rapidly after birth but begin decreasing prenatally starting at the end of the first trimester. Normal adult levels are usually achieved by the age of 8 to 12 months. The function of AFP in adult humans is unknown; however, in rodents it binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. As human AFP does not bind estrogen, its function in humans is less clear.
  • Human Alpha-fetoprotein Protein information
  • Uniprot ID FETA_HUMAN
  • Uniprot AC P02771; B2RBU3;
  • UniGene Hs.518808;
  • GeneID 174
  • KEGG hsa:174;
  • Human Alpha-fetoprotein Protein SEQUENCE
  • SEQUENCE 609 AA; 68678 MW; 4D4E45820E1C2D4F CRC64;

    MKWVESIFLI FLLNFTESRT LHRNEYGIAS ILDSYQCTAE ISLADLATIF

    FAQFVQEATY KEVSKMVKDA LTAIEKPTGD EQSSGCLENQ LPAFLEELCH

    EKEILEKYGH SDCCSQSEEG RHNCFLAHKK PTPASIPLFQ VPEPVTSCEA

    YEEDRETFMN KFIYEIARRH PFLYAPTILL WAARYDKIIP SCCKAENAVE

    CFQTKAATVT KELRESSLLN QHACAVMKNF GTRTFQAITV TKLSQKFTKV

    NFTEIQKLVL DVAHVHEHCC RGDVLDCLQD GEKIMSYICS QQDTLSNKIT

    ECCKLTTLER GQCIIHAEND EKPEGLSPNL NRFLGDRDFN QFSSGEKNIF

    LASFVHEYSR RHPQLAVSVI LRVAKGYQEL LEKCFQTENP LECQDKGEEE

    LQKYIQESQA LAKRSCGLFQ KLGEYYLQNA FLVAYTKKAP QLTSSELMAI

    TRKMAATAAT CCQLSEDKLL ACGEGAADII IGHLCIRHEM TPVNPGVGQC

    CTSSYANRRP CFSSLVVDET YVPPAFSDDK FIFHKDLCQA QGVALQTMKQ

    EFLINLVKQK PQITEEQLEA VIADFSGLLE KCCQGQEQEV CFAEEGQKLI

    SKTRAALGV

  • UCSC uc003hgz.3; human.;




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