Human AFP ELISA Kit

Alternative name
Human alpha-1-fetoprotein ELISA Kit;Human alpha-fetoglobulin ELISA Kit;Human HPAFP ELISA Kit;Human AFPD ELISA Kit;Human FETA ELISA Kit;Human alpha fetoprotein ELISA Kit;Human alpha-fetoprotein ELISA Kit;
Catalog
E001147
species
Human
GeneAFP
Standard Curve
Other Species Human AFP-L3 ELISA Kit Human AFP L1 ELISA Kit Human AFP L2 ELISA Kit Human AFP-L2 ELISA Kit Human AFP.L3 ELISA Kit Human AFP-L1 ELISA Kit Mouse Afp ELISA Kit Nude Mouse AFP ELISA Kit Mouse AFP-L2 ELISA Kit Mouse AFP-L1 ELISA Kit Mouse AFP-L3 ELISA Kit Bovine AFP ELISA Kit Canine AFP ELISA Kit Chicken AFP ELISA Kit Horse AFP ELISA Kit Porcine AFP ELISA Kit Rat Afp ELISA Kit
SpecificityNatural and recombinant human AFP
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Sample For quantitative detection of human AFP in cell culture supernates, serum and plasma
Intended UseHuman AFP ELISA Kit allows for the in vitro quantitative determination of AFP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: Human AFP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for AFP has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: R19-V609) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for AFP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human AFP amount of sample captured in plate. Background: Alpha-fetoprotein (AFP, alpha-fetoprotein; also sometimes called alpha-1-fetoprotein, alpha-fetoglobulin, or alpha fetal protein) is a protein that in humans is encoded by the AFP gene. The AFP gene is located on the q arm of chromosome 4 (4q25). AFP is the most abundant plasma protein found in the human fetus. Plasma levels decrease rapidly after birth but begin decreasing prenatally starting at the end of the first trimester. Normal adult levels are usually achieved by the age of 8 to 12 months. The function of AFP in adult humans is unknown; however, in rodents it binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. As human AFP does not bind estrogen, its function in humans is less clear.
Human Alpha-fetoprotein Protein information
Uniprot ID FETA_HUMAN
Uniprot AC P02771; B2RBU3;
UniGene Hs.518808;
GeneID 174
KEGG hsa:174;
Human Alpha-fetoprotein Protein SEQUENCE
SEQUENCE 609 AA; 68678 MW; 4D4E45820E1C2D4F CRC64;
MKWVESIFLI FLLNFTESRT LHRNEYGIAS ILDSYQCTAE ISLADLATIF
FAQFVQEATY KEVSKMVKDA LTAIEKPTGD EQSSGCLENQ LPAFLEELCH
EKEILEKYGH SDCCSQSEEG RHNCFLAHKK PTPASIPLFQ VPEPVTSCEA
YEEDRETFMN KFIYEIARRH PFLYAPTILL WAARYDKIIP SCCKAENAVE
CFQTKAATVT KELRESSLLN QHACAVMKNF GTRTFQAITV TKLSQKFTKV
NFTEIQKLVL DVAHVHEHCC RGDVLDCLQD GEKIMSYICS QQDTLSNKIT
ECCKLTTLER GQCIIHAEND EKPEGLSPNL NRFLGDRDFN QFSSGEKNIF
LASFVHEYSR RHPQLAVSVI LRVAKGYQEL LEKCFQTENP LECQDKGEEE
LQKYIQESQA LAKRSCGLFQ KLGEYYLQNA FLVAYTKKAP QLTSSELMAI
TRKMAATAAT CCQLSEDKLL ACGEGAADII IGHLCIRHEM TPVNPGVGQC
CTSSYANRRP CFSSLVVDET YVPPAFSDDK FIFHKDLCQA QGVALQTMKQ
EFLINLVKQK PQITEEQLEA VIADFSGLLE KCCQGQEQEV CFAEEGQKLI
SKTRAALGV
UCSC uc003hgz.3; human.;

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Category

Human AFP ELISA Kit

Human alpha-1-fetoprotein ELISA Kit;Human alpha-fetoglobulin ELISA Kit;Human HPAFP ELISA Kit;Human AFPD ELISA Kit;Human FETA ELISA Kit;Human alpha fetoprotein ELISA Kit;Human alpha-fetoprotein ELISA Kit;