Human IL 25 ELISA Kit

Contact Us
  • Alternative name

    Interleukin-25 ELISA KIT; Interleukin-17E ELISA KIT; IL-17EIL25 ELISA KIT; IL17E ELISA KIT; UNQ3120/PRO10272 ELISA KIT; IL-25 ELISA KIT; IL-17E ELISA KIT

  • Catalog
  • species
  • GeneIL 25
  • Standard CurveHuman IL 25 ELISA Kit
  • Other Species Human IL-17E/IL-25 ELISA KitHuman IL-25 ELISA KitMouse IL-25 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman IL 25 ELISA Kit allows for the in vitro quantitative determination of IL 25 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    Intended Uses: This IL-25 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IL-25. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: IL-25 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-25 antibody and an IL-25-HRP conjugate. The assay sample and buffer are incubated together with IL-25-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-25 concentration since IL-25 from samples and IL-25-HRP conjugate compete for the anti-IL-25 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-25 from the sample, fewer sites are left to bind IL-25-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-25 concentration in each sample is interpolated from this standard curve.

Inquiry About Human IL 25 ELISA Kit If you hope to order it or contact us directly, please contact us via We will get back to you in 12hrs,Thanks!