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Human PLA2G4A ELISA Kit

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  • Alternative name

    Human cPLA2 ELISA Kit;Human phospholipase A2 group IVA ELISA Kit;Human phosphatidylcholine 2-acylhydrolase ELISA Kit;Human PLA2G4 ELISA Kit;Human cPLA2-alpha ELISA Kit;Human cytosolic phospholipase A2 ELISA Kit;Human calcium-dependent phospholipid-binding protein ELISA Kit;Human lysophospholipase ELISA Kit;Human phospholipase A2, group IVA (cytosolic, calcium-dependent) ELISA Kit;
  • Catalog
    E011270
  • species
    Human
  • GenePLA2G4A
  • Other Species Mouse Pla2g4a ELISA KitBovine PLA2G4A ELISA KitChicken PLA2G4A ELISA KitHorse PLA2G4A ELISA KitRabbit PLA2G4A ELISA KitRat Pla2g4a ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseHuman PLA2G4A ELISA Kit allows for the in vitro quantitative determination of PLA2G4A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-cPLA2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-cPLA2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the cPLA2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of cPLA2 can be calculated.
  • Human Lysophospholipase Protein information
  • Uniprot ID PA24A_HUMAN
  • Uniprot AC P47712; B1AKG4; Q29R80;
  • UniGene Hs.497200;
  • GeneID 5321
  • KEGG hsa:5321;
  • Human Lysophospholipase Protein SEQUENCE

  • SEQUENCE 749 AA; 85239 MW; EE71CA0EBE617856 CRC64;

    MSFIDPYQHI IVEHQYSHKF TVVVLRATKV TKGAFGDMLD TPDPYVELFI

    STTPDSRKRT RHFNNDINPV WNETFEFILD PNQENVLEIT LMDANYVMDE

    TLGTATFTVS SMKVGEKKEV PFIFNQVTEM VLEMSLEVCS CPDLRFSMAL

    CDQEKTFRQQ RKEHIRESMK KLLGPKNSEG LHSARDVPVV AILGSGGGFR

    AMVGFSGVMK ALYESGILDC ATYVAGLSGS TWYMSTLYSH PDFPEKGPEE

    INEELMKNVS HNPLLLLTPQ KVKRYVESLW KKKSSGQPVT FTDIFGMLIG

    ETLIHNRMNT TLSSLKEKVN TAQCPLPLFT CLHVKPDVSE LMFADWVEFS

    PYEIGMAKYG TFMAPDLFGS KFFMGTVVKK YEENPLHFLM GVWGSAFSIL

    FNRVLGVSGS QSRGSTMEEE LENITTKHIV SNDSSDSDDE SHEPKGTENE

    DAGSDYQSDN QASWIHRMIM ALVSDSALFN TREGRAGKVH NFMLGLNLNT

    SYPLSPLSDF ATQDSFDDDE LDAAVADPDE FERIYEPLDV KSKKIHVVDS

    GLTFNLPYPL ILRPQRGVDL IISFDFSARP SDSSPPFKEL LLAEKWAKMN

    KLPFPKIDPY VFDREGLKEC YVFKPKNPDM EKDCPTIIHF VLANINFRKY

    RAPGVPRETE EEKEIADFDI FDDPESPFST FNFQYPNQAF KRLHDLMHFN

    TLNNIDVIKE AMVESIEYRR QNPSRCSVSL SNVEARRFFN KEFLSKPKA

  • UCSC uc001gsc.4; human.;



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