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Alternative name
Beta-lactamase ELISA KIT; CTX M-15 ELISA KIT;
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Catalog
E004772
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species
Human
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Genebeta-CTx
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SpecificityThis kit recognizes natural and recombinant Human beta-CTx. No significant cross-reactivity or interference between Human beta-CTx and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity75pg/mL
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Intended UseHuman beta-CTx ELISA Kit allows for the in vitro quantitative determination of beta-CTx , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses: This ELISA kit applies to the in vitro quantitative determination of Human beta-CTx concentrations in serum, plasma and other biological fluids.
Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with beta-CTx. During the reaction, beta-CTx in the sample or standard competes with a fixed amount of beta-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to beta-CTx. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of beta-CTx in the samples is then determined by comparing the OD of the samples to the standard curve.
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