Human ADAM17 ELISA Kit

Alternative name
Human ADAM 17 ELISA Kit;Human snake venom-like protease ELISA Kit;Human TNF-alpha convertase ELISA Kit;Human TNF-alpha-converting enzyme ELISA Kit;Human CSVP ELISA Kit;Human TACE ELISA Kit;Human ADAM18 ELISA Kit;Human CD156B ELISA Kit;Human NISBD ELISA Kit;Human NISBD1 ELISA Kit;Human ADAM metallopeptidase domain 17 ELISA Kit;Human disintegrin and metalloproteinase domain-containing protein 17 ELISA Kit;Human ADAM metallopeptidase domain 18 ELISA Kit;Human TNF-alpha converting enzyme ELISA Kit;Human tumor necrosis factor, alpha, converting enzyme ELISA Kit;
Catalog
E000011
species
Human
GeneADAM17
Standard Curve
Other Species Mouse Adam17 ELISA Kit Porcine ADAM17 ELISA Kit Rat Adam17 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 17 (ADAM17). No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 17 (ADAM17) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.064ng/mL.
Intended UseHuman ADAM17 ELISA Kit allows for the in vitro quantitative determination of ADAM17 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 17 (ADAM17). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 17 (ADAM17). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 17 (ADAM17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloprotease 17 (ADAM17) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Disintegrin and metalloproteinase domain-containing protein 17 Protein information
Uniprot ID ADA17_HUMAN
Uniprot AC P78536; O60226;
UniGene Hs.404914; Hs.570189; Hs.744980;
GeneID 6868
KEGG hsa:6868;
Human Disintegrin and metalloproteinase domain-containing protein 17 Protein SEQUENCE
SEQUENCE 824 AA; 93021 MW; 5B1032F6B88A837F CRC64;
MRQSLLFLTS VVPFVLAPRP PDDPGFGPHQ RLEKLDSLLS DYDILSLSNI
QQHSVRKRDL QTSTHVETLL TFSALKRHFK LYLTSSTERF SQNFKVVVVD
GKNESEYTVK WQDFFTGHVV GEPDSRVLAH IRDDDVIIRI NTDGAEYNIE
PLWRFVNDTK DKRMLVYKSE DIKNVSRLQS PKVCGYLKVD NEELLPKGLV
DREPPEELVH RVKRRADPDP MKNTCKLLVV ADHRFYRYMG RGEESTTTNY
LIELIDRVDD IYRNTSWDNA GFKGYGIQIE QIRILKSPQE VKPGEKHYNM
AKSYPNEEKD AWDVKMLLEQ FSFDIAEEAS KVCLAHLFTY QDFDMGTLGL
AYVGSPRANS HGGVCPKAYY SPVGKKNIYL NSGLTSTKNY GKTILTKEAD
LVTTHELGHN FGAEHDPDGL AECAPNEDQG GKYVMYPIAV SGDHENNKMF
SNCSKQSIYK TIESKAQECF QERSNKVCGN SRVDEGEECD PGIMYLNNDT
CCNSDCTLKE GVQCSDRNSP CCKNCQFETA QKKCQEAINA TCKGVSYCTG
NSSECPPPGN AEDDTVCLDL GKCKDGKCIP FCEREQQLES CACNETDNSC
KVCCRDLSGR CVPYVDAEQK NLFLRKGKPC TVGFCDMNGK CEKRVQDVIE
RFWDFIDQLS INTFGKFLAD NIVGSVLVFS LIFWIPFSIL VHCVDKKLDK
QYESLSLFHP SNVEMLSSMD SASVRIIKPF PAPQTPGRLQ PAPVIPSAPA
APKLDHQRMD TIQEDPSTDS HMDEDGFEKD PFPNSSTAAK SFEDLTDHPV
TRSEKAASFK LQRQNRVDSK ETEC
UCSC uc002qzu.5; human. [P78536-1];

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Human ADAM17 ELISA Kit