Human CETP ELISA Kit

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  • Alternative name

    Human lipid transfer protein I ELISA Kit;Human BPIFF ELISA Kit;Human HDLCQ10 ELISA Kit;Human cholesteryl ester transfer protein ELISA Kit;Human BPI fold containing family F ELISA Kit;Human cholesteryl ester transfer protein plasma ELISA Kit;

  • Catalog
    E008226
  • species
    Human
  • GeneCETP
  • Standard CurveHuman CETP ELISA Kit
  • Other Species Human CETP/LTP ELISA KitMouse CETP/LTP ELISA KitMouse CETP ELISA KitChicken CETP ELISA KitHamster CETP ELISA KitMonkey CETP ELISA KitRabbit CETP ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CETP. No significant cross-reactivity or interference between CETP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CETP and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CETP ELISA Kit allows for the in vitro quantitative determination of CETP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the assay: CETP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CETP. Standards or samples are then added to the microtiter plate wells and CETP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CETP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CETP are added to each well to "sandwich" the CETP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CETP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CETP concentration in each sample is interpolated from this standard curve.
  • Human Cholesteryl ester transfer protein Protein information
  • Uniprot ID CETP_HUMAN
  • Uniprot AC P11597; Q13987; Q13988; Q53YZ1;
  • UniGene Hs.89538;
  • GeneID 1071
  • KEGG hsa:1071;
  • Human Cholesteryl ester transfer protein Protein SEQUENCE
  • SEQUENCE 493 AA; 54756 MW; CD7762766A9B062E CRC64;

    MLAATVLTLA LLGNAHACSK GTSHEAGIVC RITKPALLVL NHETAKVIQT

    AFQRASYPDI TGEKAMMLLG QVKYGLHNIQ ISHLSIASSQ VELVEAKSID

    VSIQNVSVVF KGTLKYGYTT AWWLGIDQSI DFEIDSAIDL QINTQLTCDS

    GRVRTDAPDC YLSFHKLLLH LQGEREPGWI KQLFTNFISF TLKLVLKGQI

    CKEINVISNI MADFVQTRAA SILSDGDIGV DISLTGDPVI TASYLESHHK

    GHFIYKNVSE DLPLPTFSPT LLGDSRMLYF WFSERVFHSL AKVAFQDGRL

    MLSLMGDEFK AVLETWGFNT NQEIFQEVVG GFPSQAQVTV HCLKMPKISC

    QNKGVVVNSS VMVKFLFPRP DQQHSVAYTF EEDIVTTVQA SYSKKKLFLS

    LLDFQITPKT VSNLTESSSE SVQSFLQSMI TAVGIPEVMS RLEVVFTALM

    NSKGVSLFDI INPEIITRDG FLLLQMDFGF PEHLLVDFLQ SLS

  • UCSC uc002eki.3; human. [P11597-1];



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