Rat VB12 ELISA Kit

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  • Alternative name

    Vitamin B12 ELISA Kit

  • Catalog
  • species
  • GeneVB12
  • Standard CurveRat VB12 ELISA Kit
  • Other Species Mouse VB12 ELISA KitBovine VB12 ELISA KitCanine VB12 ELISA KitChicken VB12 ELISA KitHuman VB12 ELISA KitGeneral VB12 ELISA KitPorcine VB12 ELISA KitRabbit VB12 ELISA KitCamel VB12 ELISA KitGoat VB12 ELISA KitGuinea Pig VB12 ELISA KitHamster VB12 ELISA KitHorse VB12 ELISA KitMonkey VB12 ELISA KitSheep VB12 ELISA Kit
  • SpecificityNo cross-reaction with other factors
  • Sensitivity0.469ng/ml
  • Intended UseRat VB12 ELISA Kit allows for the in vitro quantitative determination of VB12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole kit at 4℃
  • Product Description
    Rat VB12 ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to VB12 , During the reaction,VB12 in the sample or standard competes with a fixed amount of biotin-labeled VB12 for sites on a pre-coated Monoclonal antibody specific to VB12 . Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VB12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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