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Alternative name
Progesterone ELISA Kit,P4 ELISA Kit
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Catalog
E103713
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species
Bovine
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GeneP4
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Standard Curve
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Other Species
Canine P4 ELISA KitChicken P4 ELISA KitHuman P4 ELISA KitGeneral P4 ELISA KitMouse P4 ELISA KitPorcine P4 ELISA KitRabbit P4 ELISA KitRat P4 ELISA KitCamel P4 ELISA KitGoat P4 ELISA KitGuinea Pig P4 ELISA KitHamster P4 ELISA KitHorse P4 ELISA KitMonkey P4 ELISA KitSheep P4 ELISA Kit
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SpecificityNo cross-reaction with other factors
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Sensitivity0.23ng/ml
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Intended UseBovine P4 ELISA Kit allows for the in vitro quantitative determination of P4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole kit at 4℃
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Product Description
specificalBovine P4 ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to P4, During the reaction,P4 in the sample or standard competes with a fixed amount of biotin-labeled P4 for sites on a pre-coated Monoclonal antibody specific to P4. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of P4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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