Sensitivity0.05 ng/mL (mean of 6 independent assays).
Intended UseHuman ASD ELISA Kit allows for the in vitro quantitative determination of ASD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses: For the quantitative detection of Human Androstenedione (ASD) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for ASD and Horseradish Peroxidase (HRP) conjugated ASD. The competitive inhibition reaction is launched between with HRP labeled ASD and unlabeled ASD with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of ASD in the sample. The color development is stopped and the intensity of the color is measured.
Background/Introduction: Androstenedione is a 19-carbon steroid hormone produced in the adrenal glands and the gonads as an intermediate step in the biochemical pathway that produces the androgen testosterone and the estrogens estrone and estradiol. Androstenedione is the common precursor of male and female sex hormones. Some androstenedione is also secreted into the plasma, and may be converted in peripheral tissues to testosterone and estrogens. In females, androstenedione is released into the blood by theca cells. The function of this is to provide androstenedione substrate for estrogen production in granulosa cells, since these cells lack 17,20 lyase required for androstenedione. Similarly, theca cells lack the enzyme aromatase required to make estrogens themselves. Thus, theca cells and granulosa cells work together to form estrogen.