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Human CRISP3 ELISA Kit

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  • Alternative name

    Human CRISP-3 ELISA Kit;Human Specific granule protein of 28 kDa ELISA Kit;Human SGP28 ELISA Kit;Human Aeg2 ELISA Kit;Human CRS3 ELISA Kit;Human dJ442L6.3 ELISA Kit;Human cysteine rich secretory protein 3 ELISA Kit;Human cysteine-rich secretory protein 3 ELISA Kit;Human specific granule protein (28 kDa) ELISA Kit;
  • Catalog
    E010226
  • species
    Human
  • GeneCRISP3
  • SpecificityNatural and recombinant human CRISP3
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 50pg/ml
  • Intended UseHuman CRISP3 ELISA Kit allows for the in vitro quantitative determination of CRISP3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: human CRISP3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CRISP3 has been precoated onto 96-well plates. Standards(NSO, N21-Y245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CRISP3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CRISP3 amount of sample captured in plate. Background: Cysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. CRISP3 expression is found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. It is a potential biomarker for prostate cancer. CRISP3 gene can be defined as an early-transcribed gene with a putative role in the pathophysiology of sjogren's syndrome. CRISP3 is present in exocrine secretions and in secretory granules of neutrophilic granulocytes and is believed to play a role in innate immunity.
  • Human Cysteine-rich secretory protein 3 Protein information
  • Uniprot ID CRIS3_HUMAN
  • Uniprot AC P54108; A8K9S1; B2R8I8; Q15512; Q3MJ82; Q53FA9; Q5JW83; Q9H108;
  • UniGene Hs.404466;
  • GeneID 10321
  • KEGG hsa:10321;
  • Human Cysteine-rich secretory protein 3 Protein SEQUENCE

  • SEQUENCE 245 AA; 27630 MW; B4DD79CB7AE9E5F9 CRC64;

    MTLFPVLLFL VAGLLPSFPA NEDKDPAFTA LLTTQTQVQR EIVNKHNELR

    RAVSPPARNM LKMEWNKEAA ANAQKWANQC NYRHSNPKDR MTSLKCGENL

    YMSSASSSWS QAIQSWFDEY NDFDFGVGPK TPNAVVGHYT QVVWYSSYLV

    GCGNAYCPNQ KVLKYYYVCQ YCPAGNWANR LYVPYEQGAP CASCPDNCDD

    GLCTNGCKYE DLYSNCKSLK LTLTCKHQLV RDSCKASCNC SNSIY

  • UCSC uc003ozs.5; human. [P54108-1];



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