SensitivityThe minimum detectable dose of human CIRBP is typically less than 3.12 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman CIRBP ELISA Kit allows for the in vitro quantitative determination of CIRBP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CIRBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CIRBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CIRBP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CIRBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Cold-inducible RNA-binding protein Protein information
Q14011; B3KT17; B4E2X2;
Human Cold-inducible RNA-binding protein Protein SEQUENCE