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Human APOA2 ELISA Kit

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  • Alternative name

    Human Apo-AII ELISA Kit;Human ApoA-II ELISA Kit;Human apolipoprotein A2 ELISA Kit;Human ProapoA-II ELISA Kit;Human Apolipoprotein A-II(1-76) ELISA Kit;Human apoAII ELISA Kit;Human apolipoprotein A-II ELISA Kit;
  • Catalog
    E003282
  • species
    Human
  • GeneAPOA2
  • Standard CurveHuman APOA2 ELISA Kit
  • Other Species Mouse Apoa2 ELISA KitBovine APOA2 ELISA KitCanine APOA2 ELISA KitHorse APOA2 ELISA KitMonkey APOA2 ELISA KitRat Apoa2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ApoA2. No significant cross-reactivity or interference between ApoA2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ApoA2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 mg/L.
  • Intended UseHuman APOA2 ELISA Kit allows for the in vitro quantitative determination of APOA2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical    Principle of the assay: ApoA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti- ApoA2 antibody and an ApoA2-HRP conjugate.The assay sample and buffer are incubated together with ApoA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ApoA2 concentration since ApoA2 from samples and ApoA2-HRP conjugate compete for the anti-ApoA2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ApoA2 from the sample, fewer sites are left to bind ApoA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoA2 concentration in each sample is interpolated from this standard curve.
  • Human Truncated apolipoprotein A-II Protein information
  • Uniprot ID APOA2_HUMAN
  • Uniprot AC P02652; B2R524;
  • UniGene Hs.237658;
  • GeneID 336
  • KEGG hsa:336;
  • Human Truncated apolipoprotein A-II Protein SEQUENCE

  • SEQUENCE 100 AA; 11175 MW; 1247868A25EC3AF2 CRC64;

    MKLLAATVLL LTICSLEGAL VRRQAKEPCV ESLVSQYFQT VTDYGKDLME

    KVKSPELQAE AKSYFEKSKE QLTPLIKKAG TELVNFLSYF VELGTQPATQ

  • UCSC uc001fzc.2; human.;


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