SpecificityThis assay has high sensitivity and excellent specificity for detection of ApoA2. No significant cross-reactivity or interference between ApoA2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ApoA2 and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman APOA2 ELISA Kit allows for the in vitro quantitative determination of APOA2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: ApoA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti- ApoA2 antibody and an ApoA2-HRP conjugate.The assay sample and buffer are incubated together with ApoA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ApoA2 concentration since ApoA2 from samples and ApoA2-HRP conjugate compete for the anti-ApoA2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ApoA2 from the sample, fewer sites are left to bind ApoA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoA2 concentration in each sample is interpolated from this standard curve.
Human Truncated apolipoprotein A-II Protein information
Human Truncated apolipoprotein A-II Protein SEQUENCE