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Catalog
C000862
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Alternative name
Platelet factor 4 CLIA KIT; C-X-C motif chemokine 4 CLIA KIT; Iroplact CLIA KIT; Oncostatin-APF4 CLIA KIT; CXCL4 CLIA KIT; SCYB4 CLIA KIT; PF-4 CLIA KIT
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GenePF4
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Other Species
Human PF4 clia kitHuman PF4 clia kitMouse PF4/CXCL4 clia kit
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Species ReactivityMouse
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SpecificityThis assay has high sensitivity and excellent specificity for detection of PF4. No significant cross-reactivity or interference between PF4 and analogues was observed.
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Detection Range2.74-2,000pg/mL. The standard curve concentrations used for the CLIA's were 2,000pg/mL, 666.67pg/mL, 222.22pg/mL, 74.07pg/mL, 24.69pg/mL, 8.23pg/mL, 2.74pg/mL.
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SensitivityThe minimum detectable dose of PF4 is typically less than 0.99pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean RLU value of 20 replicates of the zero standard added by their two standard deviations.
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Product Description specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PF4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to PF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the PF4 level in the sample or standard.
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