Annexin A1 CLIA KIT; Annexin I CLIA KIT; Annexin-1 CLIA KIT; Calpactin II CLIA KIT; Calpactin-2 CLIA KIT; Chromobindin-9 CLIA KIT; Lipocortin I CLIA KIT; Phospholipase A2 inhibitory protein CLIA KIT; p35ANXA1 CLIA KIT; ANX1 CLIA KIT; LPC1 CLIA KIT
SpecificityThis assay has high sensitivity and excellent specificity for detection of Annexin A1 (ANXA1).
No significant cross-reactivity or interference between Annexin A1 (ANXA1) and analogues was observed.
SamplesSerum, plasma and other biological fluids
SensitivityThe minimum detectable dose of this kit is typically less than 0.12ng/mL.
PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A1 (ANXA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A1 (ANXA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Annexin A1 (ANXA1) level in the sample or standard.