Human HSP90B1 ELISA Kit

Alternative name
Human 94 kDa glucose-regulated protein ELISA Kit;Human GRP-94 ELISA Kit;Human heat shock protein 90 kDa beta member 1 ELISA Kit;Human tumor rejection antigen 1 ELISA Kit;Human gp96 homolog ELISA Kit;Human GRP94 ELISA Kit;Human TRA1 ELISA Kit;Human ECGP ELISA Kit;Human GP96 ELISA Kit;Human HEL-S-125m ELISA Kit;Human HEL35 ELISA Kit;Human heat shock protein 90 beta family member 1 ELISA Kit;Human endoplasmin ELISA Kit;Human endothelial cell (HBMEC) glycoprotein ELISA Kit;Human epididymis luminal protein 35 ELISA Kit;Human epididymis secretory sperm binding protein Li 125m ELISA Kit;Human heat shock protein 90kDa beta (Grp94), member 1 ELISA Kit;Human heat shock protein 90kDa beta family member 1 ELISA Kit;Human stress-inducible tumor rejection antigen gp96 ELISA Kit;Human tumor rejection antigen (gp96) 1 ELISA Kit;
Catalog
E018034
species
Human
GeneHSP90B1
SpecificityThis assay has high sensitivity and excellent specificity for detection of HSP90B1. No significant cross-reactivity or interference between HSP90B1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HSP90B1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman HSP90B1 ELISA Kit allows for the in vitro quantitative determination of HSP90B1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: HSP90B1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HSP90B1 antibody and an HSP90B1-HRP conjugate. The assay sample and buffer are incubated together with HSP90B1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSP90B1 concentration since HSP90B1 from samples and HSP90B1-HRP conjugate compete for the anti-HSP90B1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSP90B1 from the sample, fewer sites are left to bind HSP90B1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP90B1 concentration in each sample is interpolated from this standard curve.
Human Endoplasmin Protein information
Uniprot ID ENPL_HUMAN
Uniprot AC P14625; Q96A97;
UniGene Hs.192374;
GeneID 7184
KEGG hsa:7184;
Human Endoplasmin Protein SEQUENCE
SEQUENCE 803 AA; 92469 MW; 9BF6705A7A2ED0D0 CRC64;
MRALWVLGLC CVLLTFGSVR ADDEVDVDGT VEEDLGKSRE GSRTDDEVVQ
REEEAIQLDG LNASQIRELR EKSEKFAFQA EVNRMMKLII NSLYKNKEIF
LRELISNASD ALDKIRLISL TDENALSGNE ELTVKIKCDK EKNLLHVTDT
GVGMTREELV KNLGTIAKSG TSEFLNKMTE AQEDGQSTSE LIGQFGVGFY
SAFLVADKVI VTSKHNNDTQ HIWESDSNEF SVIADPRGNT LGRGTTITLV
LKEEASDYLE LDTIKNLVKK YSQFINFPIY VWSSKTETVE EPMEEEEAAK
EEKEESDDEA AVEEEEEEKK PKTKKVEKTV WDWELMNDIK PIWQRPSKEV
EEDEYKAFYK SFSKESDDPM AYIHFTAEGE VTFKSILFVP TSAPRGLFDE
YGSKKSDYIK LYVRRVFITD DFHDMMPKYL NFVKGVVDSD DLPLNVSRET
LQQHKLLKVI RKKLVRKTLD MIKKIADDKY NDTFWKEFGT NIKLGVIEDH
SNRTRLAKLL RFQSSHHPTD ITSLDQYVER MKEKQDKIYF MAGSSRKEAE
SSPFVERLLK KGYEVIYLTE PVDEYCIQAL PEFDGKRFQN VAKEGVKFDE
SEKTKESREA VEKEFEPLLN WMKDKALKDK IEKAVVSQRL TESPCALVAS
QYGWSGNMER IMKAQAYQTG KDISTNYYAS QKKTFEINPR HPLIRDMLRR
IKEDEDDKTV LDLAVVLFET ATLRSGYLLP DTKAYGDRIE RMLRLSLNID
PDAKVEEEPE EEPEETAEDT TEDTEQDEDE EMDVGTDEEE ETAKESTAEK
DEL

Category

Human HSP90B1 ELISA Kit