Human AP12 clia kit

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  • Catalog
    C003749
  • GeneAP12
  • Species ReactivityHuman
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Apelin 12 (AP12). No significant cross-reactivity or interference between Apelin 12 (AP12) and analogues was observed.
  • SamplesSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Detection Range62.5-16000pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 22.8pg/mL.
  • PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apelin 12 (AP12) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apelin 12 (AP12) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
  • Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with a monoclonal antigen. A competitive inhibition reaction is launched between biotin labeled Apelin 12 (AP12) and unlabeled Apelin 12 (AP12) (Standards or samples) with the pre-coated secondary antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Apelin 12 (AP12) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Apelin 12 (AP12) level in the sample or standard.


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