Human CRT ELISA Kit

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  • Alternative name

    Calreticulin ELISA KIT; CRP55 ELISA KIT; Calregulin ELISA KIT; Endoplasmic reticulum resident protein 60 ELISA KIT; ERp60 ELISA KIT; HACBP ELISA KIT; grp60CALR ELISA KIT; CRTC ELISA KIT; ERp60 ELISA KIT

  • Catalog
    E006244
  • species
    Human
  • GeneCRT
  • Standard CurveHuman CRT ELISA Kit
  • Other Species Mouse CRT ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Calreticulin (CRT). No significant cross-reactivity or interference between Calreticulin (CRT) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.264ng/mL.
  • Intended UseHuman CRT ELISA Kit allows for the in vitro quantitative determination of CRT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calreticulin (CRT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calreticulin (CRT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calreticulin (CRT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calreticulin (CRT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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